基质金属蛋白酶在钙化主动脉瓣中的表达及意义  

The expression and significance of metalloproteinase in the calcific aortic valve

作　　者：赵建峰[1] 祁国奇[1] 张志刚 刘苏[1] 赵宏[1] 姚玮[1] 

机构地区：[1]河北医科大学第二医院心脏外科,石家庄050000 [2]河北省沧州市人民医院病理科

出　　处：《中国综合临床》2008年第10期1002-1004,共3页Clinical Medicine of China

基　　金：河北省科技厅攻关项目指导课题（042761513）

摘　　要：目的研究基质金属蛋白酶-2（MMP-2）及其组织抑制物（TIMP-2）在退行性钙化主动脉瓣中的表达及意义。方法对15例钙化主动脉瓣膜及10例非钙化瓣膜进行免疫组化染色（sP法）及光镜电镜观察，观察MMP-2及TIMP-2的表达情况及瓣膜形态学改变。结果钙化组MMP-2及TIMP-2表达均明显高于非钙化组（均P〈0．01），细胞外基质及内皮下可见大量棕黄色颗粒，非钙化瓣膜仅可见散在的棕黄色颗粒分散在细胞外基质中。电镜下钙化瓣膜内皮损伤，间质钙化明显。结论MMP-2及TIMP-2的表达在瓣膜钙化中起重要作用，其不仅影响基质退变还参与基质的重构，但其在钙化瓣膜中的活化和调节机制尚不清楚，有待更进一步研究。Objective To study the expression and significance of metalloproteinase 2 （MMP-2） and tisse inhibitor of metalloproteinase （TIMP-2） in the degenerative calcific aortic valve. Methods 15 calcific and 10 noncalcific aortic valve acquired through aortic valve replacement,were studied by immunohistochemistry for MMP-2 and TIMP-2. Morphologic changes were observed by light and transmission electron microscopic. Results The expression of MMP-2 and TIMP-2 in calcific group were significantly higher than in non-calcific group（P 〈 0.01 ）. Positive ceils were mostly localized subendothelial and the extracellular matrix. Control valves exhibited very weak staining of MMP-2 and TIMP-2 mostly localized in the extracelluar matrix. Various degree of endothelial damage and interstitial calcification were observed. Conculsion The expression of MMP-2 and TIMP-2 may play an important role in the calcific aortic valve. They may be involved not only in matrix degradation but also in matrix remodeling. The mechanism of rogulation and activation remain to be elucidated.

关 键 词：主动脉瓣钙化 基质金属蛋白酶 免疫组织化学 

分 类 号：R542.5[医药卫生—心血管疾病]