HPLC法测定人血浆中伪麻黄碱浓度方法的改进  

An Improved HPLC Method for the Determination of Pseudoephedrine in Human Plasma

作　　者：邹静[1] 吴正中[1] 童荣生[1] 余继英[1] 陈璐[1] 

机构地区：[1]四川省医学科学院/四川省人民医院药剂科,成都市610041

出　　处：《中国药房》2008年第26期2028-2030,共3页China Pharmacy

摘　　要：目的：建立以改进的高效液相色谱法测定人血浆中伪麻黄碱浓度的方法。方法：人血浆标本以盐酸苯丙醇胺为内标，经碱化和正己烷-二氯甲烷-异丙醇（10：5：0．5）提取后，再以0．5％盐酸萃取，取下层有机相进样分析，其中色谱柱为Diamosil C18，流动相为0．2％磷酸-乙腈：9：1，流速为1mL·min^-1，柱温为30℃，检测波长为205nm。结果：伪麻黄碱血浆浓度在3～1000μg·L^-1。范围内线性关系良好（r=0．9991），最低检测限为1μg·L^-1；4种浓度的日内RSD均〈7％，日间RSD均〈8％；平均萃取回收率为81．9％。结论：本方法简便、快速、准确，可用于人体内伪麻黄碱的血药浓度监测及药动学研究。OBJECTIVE： To determine the concentration of pseudoephedrine （PSE） in human plasma by an improved HPLC method. METHODS： With Phenylpropanolamine HCI as internal standard, human plasma samples were alkalified and extracted with n- hexane- dichloromethane- isopropyl alcohol （10 ： 5 ： 0.5）, then extracted with 0.5% HCI. The underlayer organic phase was analyzed on Diamosil C18 with mobile phase consisted of 0.2% phosphoric acid - acetonitrile （9 ： 1） at a flow - rate of 1 mL · min^ -1. The column temperature was set at 30 12 and the detection wavelength was set at 205 nm. RE- SULTS： The concentration of PSE was 3--1 000μg · L^-1（r = 0.999 1） with the lowest detectable limit at 1μg · L^-1. At 4 concentrations, the intraday RSD were all less than 7% and the interday RSD of PSE were all less than 8%. The mean recovery rate for PSE was 81.9%. CONCLUSIONS： The method is simple, rapid, accurate, and suitable for pharmacokinetic study and monitoring of serum concentration of PSE.

关 键 词：伪麻黄碱 高效液相色谱法 血药浓度 

分 类 号：R971.1[医药卫生—药品] R969.1[医药卫生—药学]