耐力训练及力竭运动后大鼠海马CA1区神经元形态学、N-甲基-D-天冬氨酸受体及突触体素的变化  

Changes of expression of glutamate and glutamate receptor NMDA and synaptophysin in rats' hippocampus after endurance training and exhaustion treadmill running

作　　者：张建军[1] 

机构地区：[1]郑州大学体育学院,郑州450044

出　　处：《郑州大学学报（医学版）》2008年第5期881-884,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:观察大鼠耐力训练及力竭运动后海马CA1区神经元形态学改变、N-甲基-D-天冬氨酸受体(NM-DA)及突触体素(synaptophysin,SYN)的变化。方法:选取8周龄雄性SD大鼠50只,随机等分为5组:安静组,急性力竭运动后即刻组,急性力竭运动后24h组,耐力训练+急性力竭运动后即刻组,耐力训练+急性力竭运动后24h组。安静组不外加运动,其他组次日进行力竭运动。力竭运动:开始速度为10m/min,逐渐提高速度并在5min内到达预定的速度,并保持速度直至力竭,并记录力竭运动时间(力竭标准为大鼠用毛刷驱赶无效,在跑台尾端停留2s仍不愿跑,且失去快速翻正反射)。耐力训练方案:大鼠在动物跑台进行运动训练,1次/d,6d/周。跑台速度由开始的10m/min逐渐增加至第4周的30m/min,运动时间由30min/d增加到40min/d。4周后断头处死取脑,检测各组大鼠海马CA1区神经元形态学改变、NMDA及SYN的变化。结果:50只SD大鼠均完成实验设计方案。运动训练后,随运动强度的增加和时间的延长,海马CA1区神经元的形态学变化显著,切片尼氏染色显示,与安静组相比,运动组锥体细胞层变薄,神经元密度明显下降,神经元胞体肿胀、变形,细胞间隙增宽,细胞排列稀疏,并有神经元崩解、丢失现象;大鼠海马NMDA受体的表达增高;SYN免疫阳性产物密度明显下降。结论:大强度的剧烈运动可引起海马NMDA受体及SYN的一系列变化,使海马神经元发生功能和形态改变,海马神经元兴奋性降低,突触传递效能下降,这可能是产生运动疲劳的中枢原因之一。Aim ： To explore the changes on expression of glutamate receptor NMDA and synaptophysin in rats＇ Hippocampus after endurance training and exhaustion treadmill running. Methods ： Fifty male SD rats of 8 weeks old were divided into 5 groups randomly with lO rats in each group： sedentary group （without training）, right after acute exercise group （without training, rats were sacrificed right after acute） ,24 hours after acute exercise group （without training, sacrificed 24 hours later after acute exhaustion treadmill running） ,right after acute exercise with training group（ performance endurance training program, rats were sacrificed right after acute exhaustion treadmill running）,24 hours after acute exercise with training group（ performance endurance training program ,rats were sacrificed 24 hours after acute exhaustion treadmill running）. Endurance training program：once a day,6 days a week. speed increased from lOm per minute at the first week to 30 m per minute at the fourth week. Training time was that from 30 minutes per day at the first week to 40 minutes per day at the fourth week. Acute exercise means an exhaustion treadmill running with the speed of 28 m per minute. The exhaustion standard was the rat could not keep the speed and stagnant stay in the 1/3 back part of the treadmill even with electricity stimulation for lO s more than 3 times. Rats were sacrificed with decollation. Hippocampus of the brain were separated were used to detect the Changes on cell apoptosis of brain and observation of pyramidal cell and mitochondria by transmission electron microscopy in rats＇ Hippocampus after endurance training and exhaustion treadmill running. Results： Totally 50 SD rats were done the experimental program and involved in the result analysis. Conclusion ： Functional transform of the hippocampus neure due to overexereise, is one of the central mechanism of exercise fatigue. Synaptie transmission activity on hippoeampus is an important index to reflect brain functional conditio

关 键 词：大鼠 耐力训练 力竭运动 N-甲基-D-天冬氨酸 突触体素 

分 类 号：G808[文化科学—体育训练] R338[文化科学—运动人体科学]