氧化苦参碱诱导结肠癌细胞株SW1116凋亡的实验研究  

Study on the apoptosis of colon cancer cell line SW1116 induced by oxymatrine

作　　者：杨道科[1] 王英俊[1] 朱洪海[1] 

机构地区：[1]郑州大学第一附属医院,河南郑州450052

出　　处：《中国医药导报》2008年第27期26-27,共2页China Medical Herald

摘　　要：目的:探讨氧化苦参碱(OM)抗肿瘤的作用机制,为OM在抗结肠癌方面的应用提供理论依据。方法:采用流式细胞仪检测不同浓度的OM对结肠癌细胞凋亡率及其细胞周期分布的影响。结果:OM具有一定的诱导结肠癌细胞凋亡的作用,可使S期细胞数目减少,随着OM剂量的加大,结肠癌细胞凋亡率增高,与剂量呈正相关。随药物浓度及作用时间的变化,细胞周期分布呈现G1期细胞比例逐渐增高。结论:OM具有一定的抗结肠癌作用,其机制与抑制癌细胞的增殖及促进癌细胞的凋亡有关。Objective： To study the anti-tumor mechanism of Oxymatrine （OM） in order to provide theoretical basis for the applications of OM in treatment of colon cancer. Methods： The effect of OM on apoptotic rate and the cell cycle distribution of colon cancer cell was detected by flow cytometry. Results： OM can cause the apoptosis ,of colon ：cancer cells in a dose-dependent manner. It can induce the amount of S phase cells and the apoptotic rate increased while the dose of OM increased, With the change of drug concentration and action time, the percentage of G1 phase changes. Conclusion： The anti-colon cancer effect of OM is related to its ability of inhibiting proliferation and promoting apoptosis of cancer ceils.

关 键 词：氧化苦参碱 结肠癌细胞株SW1116 凋亡 中药药理 

分 类 号：R961[医药卫生—药理学]