HPLC测定毛果杜鹃中槲皮苷的含量  

Determination of Quercitrin in Rhododendron seniavinii Maxim.by HPLC

作　　者：卢端萍[1] 

机构地区：[1]福建省药品检验所,福州350001

出　　处：《中国现代中药》2008年第9期14-15,共2页Modern Chinese Medicine

摘　　要：目的:测定毛果杜鹃中槲皮苷的含量。方法:采用HPLC,用Hypersil C_(18)色谱柱,以乙腈-0.05%磷酸(20:80)为流动相,检测波长350nm。结果:槲皮苷在1.966～98.3μg·mL^(-1)线性关系良好,槲皮苷平均回收率为100.30%,RSD为2.54%(n=6)结论:该方法简便快速,准确实用,可用于控制毛果杜鹃的质量。Objective： To establish the method of determining quercitrin in Rhododendron seniavinii Maxim. by HPLC. Methods： HPLC was adopted and its conditions were as follows： Hypersil C18 column, the mobile phase of acetonitrile-0. 05% phosphoric acid （20： 80）, detection wavelength was at 350nm. Results： The linear range of quercitrin was 1. 966 -98. 3ug·mL^-1. The average recovery of quercitrin was 100. 30%, RSD was2. 54% （n =6） . Conclusion： This method is simple, accurate. It can be used to control the quality of Rhododendron seniavinii Maxim.

关 键 词：HPLC 毛果杜鹃 槲皮苷 

分 类 号：R927.2[医药卫生—药学] R282.5