干旱对不同抗旱性小麦幼苗超氧物歧化酶的影响  

The Effect of Drought on Superoxide Dismutase in Seedlings of Wheat Cultivars with Different Drought Resistance

作　　者：崔四平[1] 李俊明[1] 魏建昆[1] 张敬贤[1] 

机构地区：[1]河北省农林科学院农业物理生理生化研究所,石家庄050051

出　　处：《华北农学报》1990年第3期9-13,共5页Acta Agriculturae Boreali-Sinica

摘　　要：不同抗旱性小麦幼苗的超氧物歧化酶(SOD)活性在细胞内分布存在差异,表现在抗旱品种的叶绿体和细胞溶质SOD活性、植株总SOD活性高于不抗旱品种,而线粒体SOD活性低于不抗旱品种.干旱处理后,SOD活性增加,抗旱品种酶活性增加值高于不抗旱品种.本研究还观察到8条SOD同工酶酶谱,其中干旱诱导胚芽细胞溶质产生一条新酶谱.The results indicated that the distribution of SOD attivity varied with organelles. For chloroplasts (1000 × g precipitate fraction) and cytoplasmic fraction (11000 ×g supernatant) the drought resistant cultivar had higher SOD activity than the drought sensitive one. But for mitochondria (11000 × g pellet fraction) , the drought sensitive cultivar had higher SOD activity. Under the drought condition, total SOD activity increased greatly in the drought resistant cultivar compared to the drought sensitive one. Polyacryla-mide gel electrophoresis showed eight SOD isoenzyme bands for wheat seedlings. A new isoenzyme band was induced by water stress in the cytoplasmic fraction of the coleoptiles (including first leaves) .

关 键 词：小麦 幼苗 干旱 超氧物 歧化酶 

分 类 号：S512.103.4[农业科学—作物学]