猪心肌非缺血性梗死研究  

Non-ischemic myocardial infarction in a swine model

作　　者：卫洪超 胡盛寿 唐跃 赵红[1] 张岩 陆敏杰 

机构地区：[1]中国医学科学院心血管病研究所阜外心血管病医院卫生部心血管再生医学重点实验室,北京100037

出　　处：《中华实验外科杂志》2008年第9期1103-1104,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30125309）

摘　　要：目的观察心肌非缺血性梗死发生发展的规律。方法中国小型猪12头，体重18-20kg，实验组结扎LAD上等流量点，对照组解剖相应部位LAD外膜（n=6），术中测定左心室心底和心尖部位血流动力学指标。4周后取出心脏标本，将左心室分成17段，4％多聚甲醛固定1周，苏木素-伊红（HE）、PATH及天狼星红染色进行病理学观察。结果结扎LAD4周后，左心室17段心肌内均可发现大小不等的梗死灶。左心室腔近心端收缩压收缩压[（264．05±49．34）比（168．35±31．74）mmHg（1mmHg=0．133kPa）]、平均压[（128．65±33．68）比（82．86±14．45）mmHg、发展压[（255．09±103．18）比（178．70±31．70）mmHg]与心尖部比较差异有统计学意义（P〈0．05）。结论结扎猪左冠状动脉前降支后，左心室非缺血区域可发生局灶性梗死。Objective To observe the development of non-ischemic myocardial infarction. Methods Twelve Chinese mini-pigs, weighing 18-20 kg, were randomly divided into the experimental group and control group （ n = 6）. LAD was ligated at site of （ 25.06 ± 2.50） ml/min in experimental group and tunica adventitia was dissected free only in control group respectively. All animals underwent serial hemodynamic studies of apical and bottom of left ventricle during operation. Four weeks after LAD ligation left ventricle myocardium was divided into 17 segments, and sections from each myocardium segment were fixed in 5 % buffered formalin, paraffin embedded, sectioned at 3 to 4 m, and stained with hematoxylin and eosin, FTAH and Picrosirius Red for pathologic observations. Results In the experimental group, there were significant increases in left ventricle systolic pressure [ （ 264.05 ± 49.34） vs （ 168.35 ± 31.74） mm Hg （ 1 mm Hg） = 0. 133 kPa） ], mean blood pressure [ （ 128.65 ±33.68） vs （ 82.86 ± 14.45 ） mm Hg ] and development pressure [ （255.09 ±103.18） vs （178.70±31.70） mm Hg] in the bottom as compared with those in the apical of left ventricle 4 weeks after LAD ligation. Analysis of the histologic sections from the 17 segments of LV, including the infarct, border zone and the remote demonstrated fibrosis consistent with healed infarction and inviable tissues occurred in all the 17 segments of myocardium. Conclusion Infracted myocardium extends to involve 17 segments of myocardium of the whole left ventricle as left ventricle remodeling after LAD ligation.

关 键 词：急性心肌梗死 医学实验 左心室 心尖 

分 类 号：R542.22[医药卫生—心血管疾病] R-33[医药卫生—内科学]