人工肋骨支架多孔磷酸钙的制备及体内降解研究  

Preparation of calcium phosphate cement for artificial rib scaffold and its degradahility in vivo

作　　者：唐华[1] 徐志飞[1] 刘昌胜[2] 秦雄[1] 赵学维[1] 薛磊[1] 

机构地区：[1]第二军医大学附属长征医院胸心外科,上海200003 [2]华东理工大学

出　　处：《中华实验外科杂志》2008年第9期1108-1109,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30571847）

摘　　要：目的对多孔磷酸钙（CPC）体内降解情况进行研究，为其作为组织工程化人工肋骨支架提供实验基础。方法将CPC材料埋植于兔皮下，并于预定的时间点将材料取出，称重并送扫描电镜检查，以观察其降解情况；其周围组织则进行苏木素一伊红（HE）染色和透射电镜检查，以观察其炎症反应和生物相容性。结果CPC材料在术后2、4、8、12、24周的生物吸收率分别为（2．500±0．098）％、（7．000±0．167）％、（10．000±0．242）％、（14．000±0．251）％、（30．000±0．177）％，降解速度平稳，能有效维持缺损部位的稳定性；HE染色和透射电镜结果显示其炎症反应于4周时消失，有良好的生物相容性。结论CPC的体内降解速度平稳，组织相容性好，是承重部位和非承重部位的良好组织工程支架。Objective In order to provide the further evidence of possibility of Calcium phosphate cement （CPC） as the suitable scaffold for tissue engineered artificial rib,the degradation of CPC in vivo was studied. Methods The CPC materials were implanted subcutaneously in rabbits. CPC materials were taken out at the scheduled time points, weighed and examined under a transmission electron microscope. CPC degradation was observed. The tissues around the CPC materials were observed by HE staining and the transmission electron microscope. Results The mass weight loss rate of CPC at 2,4,8,12,24 week was （2. 500 ±0. 098 ） % , （ 7. 000 ± 0. 167 ） % , （ 10. 000 ± 0. 242 ） % , （ 14. 000 ±0. 251 ） % and （30.000 ±0. 177 ）% respectively. The speed of degradation was stable. The results of HE staining and transmission electron microscopy revealed that the CPC material had a good compatibility with body. Conclusion CPC is a suitable scaffold for tissue engineered artificial rib.

关 键 词：肋骨 支架 磷酸钙 组织工程 

分 类 号：R318.1[医药卫生—生物医学工程]