小鼠深静脉血栓形成及再通模型的建立和评价  

Establishment and evaluation of deep venous thrombosis and recanalization model in mice

作　　者：邓素雄[1] 刘龙山[1] 马毅[1] 王长希[1] 

机构地区：[1]中山大学附属第一医院器官移植中心,广州510080

出　　处：《中华实验外科杂志》2008年第9期1206-1208,F0004,共4页Chinese Journal of Experimental Surgery

基　　金：广东省自然科学基金资助项目（05300771）

摘　　要：目的建立和评价小鼠深静脉血栓形成及再通模型。方法采用C57／BL6小鼠，用环形结扎不完全阻断下腔静脉并用微血管夹钳夹损伤静脉壁的方法诱导深静脉血栓形成。于术后第7、14天行血管造影观察血栓再通情况；取从结扎处到髂总静脉分叉的下腔静脉和血栓，计算质量长度比，测定血栓面积；测定尿激酶型纤溶酶原激活物（u—PA）、基质金属蛋白酶（PAI-1）及纤溶酶原激活物抑制因子（MMP）-2、MMP-9的mRNA和蛋白水平的表达。结果术后第7天，80％小鼠完全性下腔静脉血栓、20％再通；术后第14天，90％小鼠血栓再通。与术后第7天比较，术后第14天血栓面积缩小80％，质量长度比减小40．7％（P〈0．05）。与对照组比，u—PA术后第7、14天表达增强，而第14天表达低于第7天（P〈0．05）；PAI-1、MMP-2和MMP-9术后第7、14天表达逐渐增强。结论用环形结扎不完全阻断下腔静脉并用微血管夹钳夹损伤静脉壁的方法可成功诱导小鼠深静脉血栓形成。研究纤溶系统和MMPs系统可分别选取术后第7、14天前后的时间点为宜。Objective To establish and evaluate the model of deep venous thrombosis （DVT） and recanalization in mice. Methods Deep venous thrombosis was induced in C57/BL6 mice by the combination of incomplete blood flow blocking of inferior caval vein （ICV） and vascular wall damage applied with a micro clip. Angiography was performed at day 7 and 14 to assess thrombus recanalization. Thrombosed ICV was harvested to calculate mass weight/length ratio,thrombus area,and to examine mRNA and protein expression of u-PA,PAI-1 ,MMP-2 and MMP-9. Results 80% complete ICV thrombosis and 20% recanalization in 10 mice were observed at day 7,and 90% mice showed recanalization at day 14. As compared to those at day 7 ,thrombus area was decreased by 80% and mass weight/length ratio decreased by 40.7% at day 14 （P 〈 0.05 ）. As compared to sham control,u-PA mRNA and protein expression was increased at day 7 and 14 ,while levels at day 14 were lower than those at day 7 （ P 〈 0.05 ）. The mRNA and protein expression of PAI-1 ,MMP-2 and MMP-9 was significantly increased at day 7 and 14. Conclusion Deep venous thrombosis in mice can be successfully induced by incomplete ligation of inferior caval vein combined with micro-clip damage of vascular wall. Around postoperative day 7 and 14 might be appropriate time points for studying respectively fibrinolytic and metalloprotease systems.

关 键 词：深静脉血栓 模型 动物 纤溶系统 基质金属蛋白酶系统 

分 类 号：R654.3[医药卫生—外科学] R-332[医药卫生—临床医学]