深低温停循环大鼠脑保护模型的建立  

Establishment of the brain protection model of deep hypothermic circulatory arrest in rats

作　　者：顾群[1] 莫绪明[1] 彭卫[1] 陈凤[1] 何晓敏[1] 丁晋阳[1] 

机构地区：[1]南京医科大学附属南京儿童医院心胸外科,210008

出　　处：《中华实验外科杂志》2008年第9期1209-1210,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30772156）;江苏省科教兴卫工程重点人才项目（2006-11）

摘　　要：目的建立一种新的深低温停循环（DHCA）大鼠脑保护模型。方法雄性SD大鼠30只，随机分为3组，即颈动脉阻断DHCA组（A组）、颈内动脉引流DHCA组（B组）和假手术组（C组）。于深低温停循环后60min时监测脑电图变化，停循环60min后恢复循环并升温。所有大鼠于术后24h处死，并取脑组织测脑含水量。结果B组大鼠仅波相对功率值明显低于A组（P〈0．01），而且两组大鼠仅波相对功率值均明显低于C组（P〈0．01）；B组大鼠θ波相对功率值明显低于A组和C组（P〈0．01），而后两组大鼠θ波相对功率差异无统计学意义。脑含水率结果显示B组大鼠脑含水量高于A组（P〈0．05）。结论颈内动脉引流DHCA模型较颈动脉阻断DHCA模型脑缺血更完全，是一种较为理想的DHCA大鼠脑保护模型。Objective To establish the brain protection model of deep hypothermic circulatory arrest （DHCA） in rats. Methods Thirty SD rats were divided randomly into three groups ： Carotid occlusion DHCA group （group A） ; inner carotid shunt DHCA group （group B） and sham-operation group （group C ）. The changes in electroencephalograph （EEG） were observed 24 h after DHCA. All rats were killed 24 h after DHCA. The brain water content was measured. Results The α and β wave relative powers of EEG in group A were lower and higher than those in group B respectively （ P 〈 0.05 ）. The brain water content was higher in group B than in group A （ P 〈 0.05 ）. Conclusion Inner carotid shunt DHCA model is a more perfect brain protection one of DHCA.

关 键 词：深低温停循环 模型 动物 脑保护 

分 类 号：R654.1[医药卫生—外科学] R285.5[医药卫生—临床医学]