冠心苏合丸原方及改进方对心肌细胞过氧化氢损伤的影响  

Effects of Radix inulae in Guanxi Suhe Pill on cardiomyocytes injury induced by H_2O_2

作　　者：李贻奎[1] 张金艳[1] 李连达[1] 

机构地区：[1]中国中医科学院西苑医院,北京100091

出　　处：《中成药》2008年第9期1279-1283,共5页Chinese Traditional Patent Medicine

基　　金：国家十五科技攻关重点项目(2004BA721A10)

摘　　要：目的：观察冠心苏合丸中土木香代替青木香后药物血清对大鼠心肌细胞过氧化氢损伤的影响，阐明冠心苏合丸系列方抗氧化自由基损伤作用机制上的异同。方法：用体外细胞培养方法，培养乳鼠心肌细胞，复制心肌细胞过氧化氢损伤模型；运用血清药理学方法，将心肌细胞与含药血清共培养，测定培养上清液中肌酸激酶（CK）活性、乳酸脱氢酶（LDH）活性、丙二醛（MDA）含量以及细胞超氧化物歧化酶（SOD）活性、Na^＋ - K^＋ -ATP酶和Ca^2＋ - Mg^2＋ -ATP酶活性。结果：含青木香的冠心苏合丸（QG）、含土木香的冠心苏合丸（TG）以及不含木香的冠心苏合丸药后30—90min的含药血清均能不同程度地降低培养上清中CK、LDH活性以及MDA含量，增强细胞SOD活性；含土木香及不含木香的两种改进冠心苏合丸方在个别指标上的作用优于含青木香的冠心苏合丸原方。结论：冠心苏合丸组方对心肌细胞氧化自由基损伤均有明显保护作用，作用机制相似；原方中青木香被土木香替换或者去掉青木香，不影响其抗氧化自由基损伤的作用。AIM： To observe the change of Guanxi Suhe Pill（GXSH） in cardiomyocytes suffering from hydro- gen peroxide-induced injury due to replacement of Aristolochia debilis sieb. et Zucc by Radix inulae. METHODS： Myocardial cells from neonatal SD rats were cultured in dulbecco＇s modified eagle＇s medium（DMEM）. The primary culture cardiomyocytes were injured by hydrogen peroxide（ 1 mmol/L） for 4 h, and serum containing GX- SH was added to the medium at the same time. The creatine kinase （CK） activity, lactate dehydrogenase （LDH） activity and malondialdehyde （MDA） content in culture medium were determined. Intracellular superoxide dismutase（SOD） activity, the activity of Na^＋ -K^＋ -ATPase and Ca^2＋ -Mg^2＋ -ATPase in the cardiomyocytes were also observed. RESULTS： GXSH containing Aristolochia debilis sieb. et Zucc, GXSH containing Radix inulae, and GXSH without above both herbs significantly inhibited the release of LDH and CK, decreased the content of MDA and increased SOD activity, increased the volume of Na^＋ -K^＋ -ATPase and Ca^2＋ -Mg^2＋ -ATPase of hydrogen peroxide-injured cardiomyocytes. The effects of.TG and BG were stronger significantly than those of QG on some parameters. CONCLUSION： GXSH all has a remarkable protective effect on cardiomyocytes suffering from hydrogen peroxide-induced injury without and with Aristolochia debilis sieb, et Zucc or taking the place of the GXSH containing Radix inulae do not influence its protective effects on cardiomyocytes suffereing from hydrogen peroxide-induced injury.

关 键 词：冠心苏合丸 心肌细胞 过氧化氢损伤 马兜铃酸 青木香 土木香 

分 类 号：R285.6[医药卫生—中药学]