还原型谷胱甘肽对缺氧缺血新生大鼠脑组织中SOD、MDA的影响  

Effects of reduced glutathione on SOD and MDA level in the brain of neonatal rat after hypoxic-ischemic brain damage

作　　者：张海涛[1] 姚笠[1] 黄素芳[1] 

机构地区：[1]哈尔滨医科大学附属第二医院儿内科,哈尔滨150086

出　　处：《中国优生与遗传杂志》2008年第9期32-33,共2页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨还原型谷胱甘肽对新生大鼠缺氧缺血性脑病脑组织超氧化物歧化酶(SOD)、丙二醛(MDA)的影响。方法将7日龄Wistar新生大鼠40只随机分为假手术组、缺氧缺血组(参照文献制作缺氧缺血模型)、还原型谷胱甘肽干预组(模型成功后阿托莫兰400mg/kg,ip)、维生素C干预组(模型成功后唯西300mg/kg,ip),各组均在规定的时间内处死动物,测定实验侧脑组织匀浆SOD和MDA含量。结果与假手术组比较,缺氧缺血组大鼠脑组织SOD含量显著降低(P<0.01),而MDA含量显著增高(P<0.01)。与缺氧缺血组比较,还原型谷胱甘肽干预组脑组织SOD含量显著增高(P<0.01),而MDA含量显著降低(P<0.01)。结论还原型谷胱甘肽能够干预缺氧缺血性脑病脑组织内自由基清除酶的活力,降低局部脂质过氧化物的含量。Objective ： To explore the effects of reduced glutathione on the level of SOD and MDA in the brain of neonatal rat after hypoxic - ischemia brain damage. Methods ： 40 seven - day - old postnatal rats Were divided into sham operation group, hypoxic - is- chemic group, reduced glutathione group and vitamin C group randomly. A hypoxic - ischemic （HI） insult was produced as previous reports. Reduced glutathione （400mg/kg） and vitamin C （300mg/kg） were administered intraperitoneally after HI insult, respective- ly. The level of SOD and MDA in brain homogenate was measured after animals were sacrificed at set time. Results： Compared with sham operation group, the level of SOD in hypoxic - ischemic group was significantly decreased （ P 〈 0. 01 ）, but the content of MDA was increased significantly （ P 〈 0.01 ） . The level of SOD was significantly enhanced （ P 〈 0.01 ） in reduced glutathione group compared to hypoxic- ischemic group, but the content of MDA was reclined significantly （ P 〈0. 01 ） . Conclusion： Reduced gluta- thione could protective the brain of neonatal rat after hypoxic -- ischemic brain damage by increasing the actiyity of SOD and decreasing the content of MDA.

关 键 词：缺氧缺血性脑病 还原型谷胱甘肽 超氧化物歧化酶 丙二醛 自由基 

分 类 号：R722.1[医药卫生—儿科]