荧光定量聚合酶链反应(FQ-PCR)定量测定尿液CMV在孕妇普查中的应用及意义  

The survey of human cytomegalocvirus in urine of pregnant women using fuorescent quantitative polymerase chain reaction

作　　者：汤洁[1] 闫素文[1] 张宁[1] 王梦醒[1] 高艳[2] 

机构地区：[1]解放军第202医院全军优生中心,沈阳110003 [2]解放军第202医院药剂科,沈阳110003

出　　处：《中国优生与遗传杂志》2008年第9期34-34,43,共2页Chinese Journal of Birth Health & Heredity

摘　　要：目的对妇产科门诊孕前、产前检查妇女尿液CMV-DNA进行普查,达到尽早发现、提前预防,避免早孕流产、孕妇CMV-DNA的垂直传播、生产过程中新生儿CMV感染的目的。方法采用FQ-PCR方法进行尿液、乳汁CMV-DNA定量测定。结果围产期孕妇尿液CMV阳性率为3.4%;孕前检查妇女尿液CMV阳性率为1.79%;产前尿CMV阳性产后乳汁CMV阳性率为35.7%。结论育龄妇女怀孕前及孕妇围产期、哺乳期和婴儿期进行CMV测定,是优生和全面提高人口素质强有力的保障。Objective： To diagnose, prevent CMV infection early and to advoid the abord and the infection of neonate from mothers, doing the survey of human cytomegalocvirus in urine of pregnant women and physical examination women before pregnancy in the obstetrics and gynecology department. Methods ： The human cytomegalocvirus in urine and milk was detected by fuorescent quantitative PCR. Results ： The positive rate of CMV - DNA in urine of pregnant women was 3.4%. The positive rate of CMV - DNA in urine of physical examination women before pregnancy was 1.79%. The positive rate of CMV - DNA in milk of these women whose was CMV - DNA positive when they were pregnant was 35.7%. Conclusion： CMV - DNA in urine or milk should be detected in the physical ex- amination women before pregnancy, the women who were pregnant or during lactation and infants. These can ensure the high quality re- production and human character.

关 键 词：人巨细胞病毒 荧光定量聚合酶链反应 尿液 普查 

分 类 号：R450[医药卫生—治疗学] R714[医药卫生—临床医学]