植物生长激素的毛细管胶束电动色谱法分离  

Separation and Determination of Plant Growth Regulators by Capillary Micellar Electrokinetic Chromatography

作　　者：袁敏[1,2] 张铭光 康经武[1,2] 李菊白 

机构地区：[1]华南师范大学化学系 [2]中国科学院兰州化学物理研究所

出　　处：《色谱》1997年第6期482-485,共4页Chinese Journal of Chromatography

基　　金：国家自然科学基金

摘　　要：以高效毛细管胶束电动色谱法对赤霉素（ＧＡ）、脱落酸（ＡＢＡ）、吲哚丁酸（ＩＢＡ）、吲哚乙酸（ＩＡＡ）、萘乙酸（ＮＡＡ）等植物生长激素的分离和测定进行了研究。考察了各种操作参数及有机添加剂对分离的影响，得到良好的分离结果。对各组分进行了定量测定研究，ＡＢＡ、ＧＡ、ＩＢＡ、ＩＡＡ及ＮＡＡ的最低检测浓度依次为５．０，３．０，０．５８，０．１５，０．１４ｍｇ／Ｌ。It is important for separation and determination of plant growth regulators in the research of plant physiology. A study of the separation of five plant growth regulators, such as abscisic acid(ABA), gibberellic acid(GA), indole butyric acid(IBA), indole acetic acid(IAA) and naphthaleneacetic(NAA) by micellar electrokinetic chromatography (MEKC) is presented. The effects of pH, SDS concentration, applied voltage and organic additives on migration behavior were investigated. Results showed that satisfactory separation was achieved on optimization of electrophoretic condition:80mmol/L SDS, 10mmol/L borate phosphate, 5% isopropanol, 18kV applied voltage, pH 80. The SDS concentration and organic additives played an important role in separating plant growth regulators, and the applied voltage and pH gave less effect. The detection limit of ABA, GA, IBA, IAA and NAA were 50×10-3,30×10-3,58×10-4,15×10-4 and 14×10-4g/L respectively. Increasing of the SDS concentration or organic additives would change separation factor(α) and result an improvement of the resolution of difficult separation pairs ABA GA and IAA NAA

关 键 词：MEKC ABA GA IBA IAA NAA 植物生长激素 

分 类 号：Q946.885[生物学—植物学]