新的多氨基多羧酸荧光螯合剂DTPA·2pAs的合成及其铽螯合物荧光性能研究  

Synthesis of a New Polyaminopolycarboxylic Acid(DTPA·2pAS) and Fluorescence of its Tb^3+ complexes in Aqueous Solution

作　　者：龚孟濂[1] 吴炜宁[1] 史华红[1] 曾春莲[1] 孟建新[1] 杨燕生[1] 

机构地区：[1]中山大学化学与化工学院

出　　处：《中国稀土学报》1997年第4期289-294,共6页Journal of the Chinese Society of Rare Earths

基　　金：国家科委攀登计划;广东省自然科学基金

摘　　要：报道二乙三胺Ｎ′，Ｎ（双）对乙酰氨基水杨酸Ｎ′，Ｎ″，Ｎ三乙酸（ＤＴＰＡ·２ｐＡＳ）的合成、表征及其敏化铽离子发光的性能。利用荧光滴定方法确定了水溶液中螯合物的组成，研究了浓度、ｐＨ对荧光强度的影响，测量了螯合物激发态的寿命，研究了ＤＴＰＡ·２ｐＡＳ从ＴｂＥＤＴＡ中取代ＥＤＴＡ引起的荧光变化。结果表明ＤＴＰＡ·２ｐＡＳ可望在荧光免疫分析中用作荧光增强溶液。 new polyaminopolycarboxylic acid, diethylenetriamineN′,Nbi(pacetoaminosalicylic acid)N′,N″,Ntriacetic acid(DTPA·2pAS), has been synthesized. DTPA·2pAS can sensitize the luminescence of Tb3+ in aqueous solution. Fluorescent titration at pH 70 indicated an 1∶1 composition of Tb3+ to DTPA·2pAS for the complexes. The complexes exhibit intensely sensitzed fluorescence in a range of pH 50～120. The fluorescence intensity of the complexes increased when the concentration was raised up from 1×10-7 to 1×10-4 mol/L, and then decreased at 1×10-3 mol/L due to concentrationquenching. A replacement of EDTA with DTPA·2pAS in Tb3+EDTA complex solution led to increasement in Tb3+ sensitized luminescence intensity, suggesting formation of Tb3+DTPA·2pAS complexes and higher stability of these complexes than those of Tb3+EDTA, which has been confirmed by determination of stability constants with pH potentiometric titration. The results show DTPA·2pAS may be applied to fluorescent immunoassay as a “fluorescenceenhancement sdution”.

关 键 词：稀土 荧光螯合剂 铽螯合物 DTAP.2pAs 

分 类 号：O657.303[理学—分析化学] O614.341[理学—化学]