硝酸钙胁迫对营养液栽培嫁接茄子叶片抗坏血酸-谷胱甘肽循环的影响  

EFFECTS OF CALCIUM NITRATE STRESS ON ASCORBATE-GLUTATHIONE CYCLE METABOLISM IN LEAVES OF HYDROPONICALLY-GROWN GRA-FTED EGGPLANT SEEDLINGS

作　　者：魏国平[1] 朱月林[1] 刘正鲁[1] 张古文[1] 杨立飞[1] 

机构地区：[1]南京农业大学园艺学院,南京210095

出　　处：《植物生态学报》2008年第5期1023-1030,共8页Chinese Journal of Plant Ecology

基　　金：教育部高校博士点基金(20030307020);江苏省科技厅资助项目(BC2003306和BE2002304)

摘　　要：以日本引进的设施专用耐盐茄(Solanum melongena)品种‘Torvum Vigor’为砧木,栽培茄(S.torvum)品种‘苏崎茄’为接穗,用营养液栽培,对80mmol·L–1Ca(NO3)2胁迫下茄子嫁接苗和自根苗叶片抗坏血酸-谷胱甘肽循环系统中抗氧化酶活性和抗氧化物及H2O2含量进行比较。结果表明,Ca(NO3)2胁迫下茄子幼苗叶片H2O2含量有所增加,但嫁接苗叶片H2O2含量显著低于自根苗。Ca(NO3)2胁迫下嫁接苗叶片抗氧化酶(APX、DHAR和GR)活性、AsA和GSH再生率、氧化还原力(AsA/DHA值和GSH/GSSG值)均显著高于自根苗。综上所述,Ca(NO3)2胁迫下嫁接苗保持良好的AsA-GSH循环效率,清除H2O2效率较高,细胞受氧化损伤程度较轻,表现出较强的耐盐性。Aims Oxidative stress is one of the responses of plants to salt stress, which causes the production of hydrogen peroxide （H2O2）. Plants grafted on salt resistant rootstocks could improve their tolerance to salt stress because of the vigorous root system of rootstocks. However, little is known about the relationship between salt tolerance of grafted plants and the scavenging system of H202 under Ca（NO3）2 stress. Our objective was to investigate the mechanism and roles of ascorbate-glutathione metabolism system in salt tolerance of grafted eggplant under Ca（NO3）2 stress, including ascorbate pool, glutathione pool and the activities of antioxidant enzymes. Methods We grafted cultivar ＇Suqi qie＇ （Solanum melongena） onto rootstock of ＇Torvum Vigor＇ （S. torvum）, which is a salt tolerant cultivar introduced from Japan. We compared leaves of hydroponi- cally-grown grafted and own-root eggplant seedlings grown under 80 mmol·L^-1 Ca（NO3）2 stress for changes of antioxidant enzyme activities, contents of antioxidants in ascorbate-glutathione cycle metabolism and content of H2O2. Important findings Under Ca（NO3）2 stress, H2O2 content increased in leaves of eggplant seedlings and was significantly lower in leaves of grafted seedlings than own-root seedlings. Also, activities of antioxidant enzymes （APX, DHAR and GR）, regenerating rates of AsA and GSH and redox statuses （ratios of AsA/DHA and GSH/GSSG） were significantly higher in leaves of grafted seedlings than own-root seedlings. We concluded that grafted eggplant seedlings had efficient metabolism of ascorbate-glutathione cycle, which scavenged the H2O2 rapidly to alleviate the oxidative damage of Ca（NO3）2 stress; therefore, grafted seedlings had a stronger tolerance to salt stress.

关 键 词：嫁接茄子 CA(NO3)2胁迫 抗坏血酸-谷胱甘肽循环 营养液栽培 

分 类 号：S641.1[农业科学—蔬菜学]