4-间氨基酚-4′去甲表鬼臼酯的抗氧化与抗肿瘤作用  

Antitumor and antioxidation action of 4-m-amino-phenol-4'-demethylepipodophyllotoxin ether

作　　者：陈晓[1] 王哲元[1] 雷洁琼[2] 张有成[1] 

机构地区：[1]兰州大学第二医院普外科,甘肃兰州730030 [2]甘肃省肿瘤医院内科,甘肃兰州730050

出　　处：《兰州大学学报（医学版）》2008年第3期1-4,共4页Journal of Lanzhou University（Medical Sciences）

摘　　要：目的研究4-间氨基酚-4′去甲表鬼臼酯(MDE)的体外抗氧化与抗肿瘤活性。方法体外对人胃癌细胞SGC-7901的抗肿瘤活性用四氮唑比色法;体内抗肿瘤活性用动物移植瘤法。用硫代巴比妥酸法测定大鼠肝自发性,Fe^(2+)-抗坏血酸诱发的心、肝、肾组织匀浆中丙二醛生成;分光光度法测定H_2O_2诱导的红细胞溶血。结果MDE剂量依赖性地抑制SGC-7901细胞生长,IC_(50)为36.5(17.1～77.8)mg/L,体内对小鼠移植性肿瘤S180和H22均有明显的抑制作用,并浓度依赖性地抑制大鼠肝组织自发性和Fe^(2+)-抗坏血酸诱导的心、肝、肾组织匀浆中丙二醛的生成,对H_2O_2诱导的大鼠红细胞溶血也有一定的抑制作用。结论MDE有明显的抗氧化及抗肿瘤作用。Objective To investigate the antitumor and antioxidation action of 4-m-aminophenol-4＇-demethylepipodophyllotoxin ether（MDE）. Methods Antitumor action was studied in vitro by MTT method, and in vivo by transplanted tumor of mice S180 and H22. Malondialdehyde generation in tissues of rats was measured with the thiobarbituric acid reaction. H2O2-induced red blood cell hemolysis was determined spectrophotometrically. Results MDE strongly inhibited SGC-7901 cell proliferation at 48 h in a concentration-dependent manner with IC50 of 36.5（17.1-77.8） mg/L. In vivo experiments showe.d that MDE obviously inhibited the growth of S180 and H22, respectively. MDE also significantly inhibited spontaneous malondialdehyde generation or malondialdehyde generation induced by Fe^2＋-ascorbic acid in homogenate of heart, liver and kidneys of rats, and hemolysis induced by H2O2. Conclusion MDE has anti-tumor and anti-oxidation action.

关 键 词：4-间氨基酚-4’去甲表鬼臼酯 抗氧化 抗肿瘤 

分 类 号：R979.1[医药卫生—药品]