大鼠慢性压迫性脊髓损伤后EphA2在脊髓前角运动神经元中的表达  

Upregulation of EphA2 in motor neurons of anterior horn of spinal cord after chronic spinal cord compressive injury in rats

作　　者：裴有智 康学文[2] 汪玉良[2] 

机构地区：[1]甘肃靖远煤业有限责任公司总医院外二科,甘肃靖远730913 [2]兰州大学第二医院骨科,甘肃兰州730030

出　　处：《兰州大学学报（医学版）》2008年第3期35-38,共4页Journal of Lanzhou University（Medical Sciences）

摘　　要：目的探讨慢性脊髓损伤后红细胞生成素诱导肝癌细胞株受体A2(EphA2)在脊髓中的变化规律,寻找脊髓损伤后治疗的靶点。方法将40只Wistar大鼠分为假手术组、轻度压迫组(CR 20%)、中度压迫组(CR 40%)、重度压迫组(CR 60%)4组。T_(10)水平脊髓背侧加压,压迫方法采用塑料螺钉渐进性加压,分次完成手术。对大鼠进行后肢运动功能评分及所取脊髓标本进行原位杂交分析,对脊髓前角EphA2阳性细胞率进行统计分析,比较各组之间的差异。结果EphA2阳性细胞主要存在于压迫远端脊髓灰质中,其中以脊髓前角运动神经元为著。假手术组有很少量EphA2阳性细胞,压迫组EphA2阳性细胞明显升高(P<0.01),中度压迫组及重度压迫组较轻度压迫组升高(P<0.05),中度压迫组及重度压迫组间差异无统计学意义(P>0.05)。结论慢性压迫性脊髓损伤后脊髓前角运动神经元EphA2表达升高。慢性脊髓压迫可以诱发脊髓EphA2表达升高,对脊髓损伤是一个保护性因素。Objective To explore the changes of erythropoietin-producing hepatoma line A2 （EphA2） in spinal cord after chronic spinal cord compression in rats. Methods Adult female Wistar rats（n=40） weighing 280-320 g were randomly divided into 4 groups, laminectomy was performed in rats of control group（n=10）. Rats in injury groups（n=30） were drilled at the root of spinous process of T10, then plastic screw was screwed by degrees, the dorsal spinal cord was compressed, models of mild, moderate and severe spinal cord injuries were made. The slices of spinal cord tissues of 5 mm from the edge of compression margin were used to observe EphA2 positive cells by in situ hybridization. Results Laminectomy group had no EphA2 positive cells; EphA2 positive cells were located in gray matter of spinal cord in injury groups, especially in large motor neurons in anterior horn. The numbers of EphA2 positive cells were significantly higher in injury groups than in laminectomy groups（P 〈0.01） and higher in moderate and severe injury groups than in mild injury group（P 〈0.05）, but no difference was found between moderate and severe injury groups（P 〉0.05）. Conclusion EphA2 is upregulated in spinal cord after chronic spinal cord compressive injury.

关 键 词：慢性压迫性脊髓损伤 红细胞生成素诱导肝癌细胞株受体A2 原位杂交 

分 类 号：R681.5[医药卫生—骨科学]