大鼠肢体缺血再灌注早期血栓前状态的研究  

Prethrombotic State in Rats after Ischemia-reperfusion of Limb and it′s Intervention Study

作　　者：杨敏[1] 马平[1] 徐文会[1] 张宏江[1] 

机构地区：[1]山西医科大学第二附属医院麻醉科,山西太原030001

出　　处：《实用骨科杂志》2008年第9期530-533,共4页Journal of Practical Orthopaedics

基　　金：山西省青年科学基金(2007021049)

摘　　要：目的探讨大鼠急性下肢缺血再灌注早期血栓的形成情况。方法将大鼠随机分为假手术组、下肢缺血再灌注组两组。夹闭大鼠股动脉并用张力带绑扎下肢,建立急性下肢缺血再灌注大鼠模型。观察急性下肢缺血再灌注大鼠血小板膜糖蛋白GP b/a和血管内皮细胞血栓调节蛋白(thrombomoclulin,TM)的表达。结果缺血再灌注组大鼠血小板膜糖蛋白GP b/a表达高于假手术组(P<0.05);缺血再灌注组大鼠血管内皮TM表达低于假手术组(P<0.05)。结论急性下肢缺血再灌注可能导致大鼠血栓前状态的形成。Objective To investigate the emerge condition of thromb in rats after ischemia-reperfusion of limb. Methods The rats were randomly divided into 2 groups：sham operation group （N group）;the hind limb ischemia reperfusion group （IR group）. An animal model of acute hind limb ischemia-reperfusion was developed by clamping femoral artery and banding hind limb of rats. Changes in the expression of GP Ⅱ b/Ⅲ a of platelet membrane and TM of vascular endothelial cell in rats after ischemia-reperfusion of limb were observed. Results The expression of GP Ⅱ b/Ⅲ a of platelet membrane in the IR group was significantly higher than that of the N group （P〈0.05）. The expression of TM of vascular endothelial cell in the IR group was significantly lower than that of the N group （P〈0.05）. Conclusion Acute hind limb ischemia-reperfusion mav induce the formation of prethrombotic state in rats.

关 键 词：肢体缺血再灌注 血栓前状态 血小板膜糖蛋白 血栓调节蛋白 

分 类 号：R554.9[医药卫生—血液循环系统疾病]