大鼠肾切除术后留存肾代偿早期细胞凋亡状态的研究  

Apoptosis in the early stage of compensatory renal growth following uninephrectomy in the young and old rats

作　　者：王行环[1,2] 顾方六[1,2] 杨宝龙[1,2] 邓方明 吴阶平[1,2] 

机构地区：[1]北京医科大学泌尿外科研究所 [2]广东省人民医院泌尿外科

出　　处：《中华医学杂志》1997年第10期742-744,共3页National Medical Journal of China

基　　金：国家自然科学基金

摘　　要：探讨调控脏器形态稳定的重要因素－细胞凋亡在肾代偿性生长中的作用。方法用荧光染色、ＤＮＡ梯度电泳分析、原位ＤＮＡ片段末端标记等凋亡检测的最新手段，系统研究青、老年大鼠正常肾及代偿肾内细胞凋亡情况的差异。结果青年正常肾无细胞凋亡表现，而老年正常肾皮、髓质小管均有细胞凋亡存在，且皮质凋亡水平高于髓质；青年肾代偿３天时细胞凋亡明显增加，且髓质高于皮质，老年肾代偿３天时，留存肾细胞凋亡水平明显降低，且以皮质降低更为明显。结论凋亡是肾代偿性生长形态学改变的重要组成部分，肾代偿性生长的年龄差异与细胞凋亡有关。Objective To investigate the role of apoptosis, one of the major factor to maintain homeostasis of cell number of organs, in the early process of compensatory renal growth, which have been considered to including cellular hyperplasia and hypertrophy, following uninephrectomy. Method DNA fragment gel electrophoresis, tissue sections photolebeling technique and in situ nick translation assay were used to analyze apoptotic cells in the compensatory and normal kidney in the young and old Wistar rats. Results Apoptosis affected normal kidney of the old group, but not the young. In the old rat′s normal kidney, apoptosis index was higher in cortical tubules than that in the medullar tubules. In the compensatory remnant kidney, apoptosis was diminished in old rats, especially in the cortex, but increased in the young rats, especially in the medulla. Conclusions Compensatory renal growth includes cellular hypertrophy, hyperplasia and apoptosis. In the old, compensatory renal growth is mainly cellular hypertrophy and reduction of cell death. In the young, compensatory renal growth is mainly cellular hyperplasia and hypertrophy, increased apoptosis may be responsible for the elimination of excess hyperplastic renal cell.

关 键 词：肾切除术 细胞凋亡 肾代偿性生长 

分 类 号：R699.2[医药卫生—泌尿科学]