谷光甘肽对冷藏枇杷果实木质化相关生理指标的影响  

Effects of Glutathione on Several Physiological Indexes Related with Lignification of Loquat Fruits during Cold Storage

作　　者：吴锦程[1] 陈伟健[2] 谭莉[1] 林德贵[1] 卢海霞[1] 

机构地区：[1]莆田学院环境与生命科学系,福建莆田351100 [2]莆田市农业检验监测中心,福建莆田351100

出　　处：《云南农业大学学报（自然科学版）》2008年第5期652-657,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：福建省科技厅资助重点课题(2007I0021);福建省教育厅资助课题(3B05228);莆田市科技局资助重点课题(2007N26)

摘　　要：以"解放钟"枇杷(Eriobotrya japonicaLindl.cv.Jiefangzhong)为试材,研究外源还原型谷胱甘肽(glu-tathione,GSH)对6℃冷藏枇杷果实苯丙氨酸解氨酶(phenylalanine ammonia-lyase,PAL)、多酚氧化酶(poly-phenol oxidase,PPO)、4-香豆酸辅酶A连接酶(4-coumarate coenzyme A ligase,4-CL)、肉桂醇脱氢酶(cin-namyl alcohol dehydrogenase,CAD)、过氧化物酶(peroxidase,POD)和多胺氧化酶(polyamine oxidase,PAO)活性以及可溶性糖含量变化与果实木质化的关系。结果表明,外源GSH处理通过抑制冷藏枇杷果实中PAL,POD和PAO活性上升,从而抑制果实木质素的形成,并延缓可溶性糖的下降;但GSH处理对果实PPO,CAD和4-CL酶活力影响较小,它们可能在枇杷果实木质化中不起主要作用。GSH具有减缓冷藏枇杷果实木质化的作用,其中以1.0 g/L和1.5 g/L的GSH所处理的枇杷果实木质化程度较低。The effects of exogenous glutathione （GSH） on lignification of loquat （ Eriobotrya japonica Lindl. cv. Jiefangzhong） fruits stored at 6 ℃ were studied. To study the relationship between lignification and changes of activities of phenylalanine ammonia lyase （ PAL, EC 4. 1. 3. 5. ）, polyphenol oxidase （PPO, EC 1. 10. 3. 2. ）, 4-coumarate coenzyme A ligase （4-CL, EC 6. 2. 1. 12. ）, cinnamyl alcohol dehydrogenase （CAD, EC 1. 1. 1. 195. ）, peroxidase （POD, EC 1. 11. 1. 7. ）, polyamine oxidase （PAO, EC 1. 4. 3. 4. ） and contents of soluble sugar, the loquat fruits were treated with GSH. The results showed that activities of PAL, POD and PAO were inhibited after the treatment with GSH. Meanwhile, the GSH treatment delayed the decomposition of soluble sugar. Consequently, the treatment with GSH inhibited the increase of lignin. However, the treatment with GSH had no significant effect on activities of PPO, 4-CL and CAD. These factors probably played insignificant roles in the lignification of loquat fruits. The results indicated that the GSH treatment could inhibit the lignification of loquat fruits under cold storage. Lower level of lignification was achieved with treatment of GSH at 1.0 g/L and 1.5 g/L, respectively.

关 键 词：枇杷 木质化 谷胱甘肽(GSH) 苯丙氨酸解氨酶(PAL) 过氧化物酶(POD) 多胺氧化酶(PAO) 

分 类 号：S667.309.3[农业科学—果树学] S791.253[农业科学—园艺学]