橡胶树死皮病黄色体蛋白质组差异分析与初步鉴定  

Differential Analysis of Two-dimensional Gel Electrophoresis Profiles and Preliminary Identification of Lutoid Proteome on the Latex of TPD in Hevea brasiliensis

作　　者：闫洁[1] 陈守才[1] 

机构地区：[1]中国热带农业科学院橡胶研究所/农业部热带作物栽培生理学重点开放实验室

出　　处：《湖北农业科学》2008年第8期858-862,共5页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(30471373)

摘　　要：应用双向凝胶电泳技术(two-dimensional gel electrophshiya/oresis,2-DE),比较橡胶树死皮株与健康株胶乳黄色体蛋白质组表达的差异。采用TCA/丙酮沉淀法提取橡胶树死皮株与健康株黄色体蛋白质,并采用固相pH梯度(immobilized pH gradient,IPG)双向凝胶电泳分离两种材料蛋白质,凝胶经银染显色后,用PDQuest图像分析软件进行比较分析、识别差异表达的蛋白质。成功获得了橡胶树死皮株与健康株胶乳黄色体的双向凝胶电泳图谱。鉴定出24个蛋白差异点,其中17个上调表达,7个下调表达。并应用质谱技术鉴定了其中部分表达差异的蛋白质点,对渗调蛋白进行了功能分析,其在死皮株中表现下调的情形可能与死皮病的发生有一定的关系。Differential proteomic analysis was conducted in lutoid proteins from latex of healthy and Tapping panel dryness （TPD） trees by two-dimension gel electrophoresis（2-DE）.The lutoid proteins from latex of healthy and TPD trees were isolated respectively by TCA/cold acetone precipitation. And these were prepared by means of immobilized pH gradient （IPG） based on 2-DE. After silver staining, the differential expression proteins of two kinds were analyzed using image analysis software （PDQuest）. Using proper method stated above, and satisfactory 2-DE maps of lutoid proteins from latex of healthy and TPD trees were obtained and the preliminary analysis results were reported. There were 24 notable spots were detected, including 17 up-regulated proteins and 7 down-regulated proteins. Of them some different-expressed protein spots were detected by mass spectrometry. There were some relationship between osmotin protein with TPD broom according to the functions of down-regulated proteins in TPD trees.

关 键 词：巴西橡胶树 死皮病 双向凝胶电泳 蛋白质组 质谱 

分 类 号：S794.1[农业科学—林木遗传育种] S763.19[农业科学—林学]