不同游泳运动方式下大鼠中央杏仁核c-fos蛋白表达的实验研究  

Experimental Research on Expression of c-fos Protein in Rats' CeA in Different-Manner Swimming

作　　者：张安民[1] 于芳[2] 胡淑萍[3] 王根深[4] 

机构地区：[1]烟台大学体育学院,山东烟台264005 [2]中北大学体育系,山西太原030051 [3]山西财经大学医院,山西太原030006 [4]山西大学体育学院,山西太原030006

出　　处：《上海体育学院学报》2008年第5期48-53,共6页Journal of Shanghai University of Sport

基　　金：国家自然科学基金资助项目(30671019)

摘　　要：目的:观察不同游泳运动方式下大鼠中央杏仁核(CeA)中c-fos蛋白表达的规律,探讨不同游泳运动方式对CeA的影响机制。方法:建立大鼠游泳运动模型(间歇运动、持续运动),采用ABC特异抗体免疫组织化学方法,观察不同游泳运动方式下大鼠CeA中c-fos蛋白表达的变化,并通过图像分析系统和统计学软件进行图像与数据分析。结果:对照组大鼠CeA中fos阳性细胞表达不明显,模型组明显增多;持续组游泳运动结束后,60 min时fos阳性细胞表达达到峰值,然后回落;间歇组峰值时刻点与持续组相同,但间歇组各时刻点c-fos蛋白表达数值均高于持续训练组,且两组峰值间具有显著性差异(P<0.05)。结论:间歇运动对于大鼠CeA中c-fos蛋白表达的影响更为显著,运动强度与c-fos蛋白表达呈正相关。The purpose： observing the law of expression of c-fos protein in rats＇ CeA in different-manner swimming and inquiring into the mechanism of effect of different-manner swimming on CeA. The methods： setting up exercise models of rats swimming （interval swimming and continuous swimming）, using the method of ABC special antibody immunochemistry to observe the changes of expression of c-fos protein in Rats＇ CeA in different-manner swimming and analyzing the data and images with picture analyzing system and statistical software. The results： the expression of c-fos positive cells in rats＇ CeA in different-manner swimming isn＇ t obvious for the control group, while it has obviously increased for the model group; the c-fos positive cells expression of the continuous-swimming group has reached peak at 60-min after exercise, and then begin to fall, which is the same as the interval-swimming group in peak-reaching time, but the latter＇s values of c-fos protein expression at different time are all higher than those of the former, and there exists significant difference between the two groups in the peak value （ P 〈 0.05 ）. The conclusions： the effect of interval swimming on the expression of c-fos protein in rats＇ CeA is more remarkable and the exercise intensity is positively correlated with the expression of c-fos protein in rats＇ CeA.

关 键 词：游泳运动 大鼠 中央杏仁核 c—fos 阳性 细胞表达 

分 类 号：G804.2[文化科学—运动人体科学]