大鼠胰腺发育中原癌基因c-met的表达及蛋白定位  

Expression and protein location of c-met in the different stages of rat pancreatic development

作　　者：程梅[1] 吴玉龙[2] 贺秀香[3] 徐仁华[1] 孙丽红[1] 

机构地区：[1]滨州医学院护理学院,山东滨州256603 [2]滨州医学院基础学院,山东滨州256603 [3]滨州医学院附属医院,山东滨州256603

出　　处：《现代生物医学进展》2008年第8期1412-1414,1418,共4页Progress in Modern Biomedicine

基　　金：教育部科学技术重点研究项目(地方01042)

摘　　要：目的:研究原癌基因c-met在大鼠胰腺发育不同阶段的表达及定位。方法:采用RT-PCR技术检测c-met基因在大鼠胰腺不同发育时期:孕15.5天(E15.5)和孕18.5天、新生、生后14天(P14)、P21及成年胰腺的表达。并用免疫组化技术对该基因编码的蛋白-肝细胞生长因子受体c-MET蛋白在胰腺发育不同阶段的定位进行分析。结果:c-met基因在E15.5、E18.5较成年特异性高表达。免疫组化结果显示该基因编码的蛋白c-MET在新生后的胰腺大量定位与胰岛细胞。结论:提示c-met可能在胰腺发育过程中起到调控作用,参与胰腺发育中新生后胰岛结构重塑过程。Objective：To investigate the expression and location of proto-oncogene c-met in the different development stages of rat pancreas.Methods：RT-PCR was used to detect the expression of c-met proto-oncogene in different development stages of rat pancreas（embryonic day 15.5,embryonic day 18.5,postnatal dayl4,postnatal day21,newborn and adult pancreas）.Immunohistochemistry was used to detect the location of the protein of c-met proto-oncogene in the different stages of rat pancreatic development.Results：c-met proto-oncogene expressed highly at rat postnatal days,specially at P14,P21of rat pancreatic development stages.Conclusion：c-met proto-oncogene may play a role in the development of rat pancreas,especially the islet remodification of postnatal rat pancreatic development.

关 键 词：C-MET 胰腺生长发育 胰岛结构重塑 RT-PCR 免疫组化 

分 类 号：R318[医药卫生—生物医学工程]