组织蛋白酶S与ApoE-/-小鼠动脉粥样硬化的实验研究  

The experimental study of Cathepsin S on atherosclerotic lesion formation in apoE-Deficient Mice

作　　者：秦彦文[1] 王绿娅[1] 叶平[2] 陈永福[1] 杨娅[1] 吕树峥[1] 

机构地区：[1]首都医科大学附属北京安贞医院-北京市心肺血管疾病研究所,北京100029 [2]中国人民解放军总医院老年心血管二科,北京100853

出　　处：《现代生物医学进展》2008年第8期1501-1503,共3页Progress in Modern Biomedicine

基　　金：北京市自然基金课题(7082020);北京市优秀人才培养资助(20071D0300600084);北京市科委重大项目资助(D0906006040191);国家自然科学基金资助(30672000)

摘　　要：目的:观察组织蛋白酶S(Cathepsin S)在不同周龄载脂蛋白E基因缺陷(ApoE-/-)小鼠主动脉的表达,初步探讨Cathepsin S对ApoE-/-小鼠动脉粥样硬化(As)病变的影响。方法:将16只8周龄ApoE-/-小鼠随机分为两组:16周龄组(n=8),24周龄组(n=8),均饲以高脂饮食,分别在16周龄和24周龄处死动物。采用普通光镜、病理图象分析法测定主动脉As斑块面积及管腔面积;免疫组织化学染色方法观察组织蛋白酶S(Cathepsin S)在不同周龄ApoE-/-小鼠主动脉的表达。结果:16周龄组ApoE-/-小鼠主动脉根部出现As病变;与16周龄组ApoE-/-小鼠相比,24周龄组ApoE-/-小鼠主动脉根部As病变显著增强(P<0.01),免疫组化显示Cathepsin S表达明显增加(P<0.01)。结论:Cathepsin S在ApoE-/-小鼠主动脉的表达随As病变程度增强而显著增加。Objective：To study the expression of Cathepsin S on the atherosclerotic lesion of aortic wall in apoE-deficienct （apoE-/-）mice of different weeks.Methods：Sixteen male apoE-deficienct mice were randomized into two groups：sixteen-week-old group（n-8）,twenty four-week-old group（n=8）.All the mice were fed with high-fat diet and killed at 16 week,24-week respectively. Optical microscopy was adopted to assess the degree of atherosclerotic of aortic wall and image analysis was performed with computer. The expression of Cathepsin S on the atherosclerotic lesion in apoE-deficienct（apoE-/-）mice of different weeks was studied by SABC immunohistochemistry and computerized with imaging analysis respectively.Results：Compared with the sixteen-week-old group,the extent of atherosclerotic lesion of twenty four-week-old group was significantly serious（P〈0.01）;the expression of Cathepsin S in aortic wall was significantly higher（P〈0.01）.Conclusion Expression of Cathepsin S is strengthened obviously along with increase of atherosclerotic lesions in apoE-deficienct mice.

关 键 词：动脉粥样硬化 组织蛋白酶S 载脂蛋白E基因缺陷鼠 

分 类 号：R543.5[医药卫生—心血管疾病]