STN抗原与产后出血关系的研究  

Study on the relationship between Sialyl Tn antigen and postpartum hemorrhage

作　　者：赵敏[1] 余舰[1] 刘华庆[2] 苏俊[2] 

机构地区：[1]遵义医学院法医学教研室,贵州遵义563003 [2]遵义医学院附属医院病理科,贵州遵义563003

出　　处：《遵义医学院学报》2008年第4期349-351,共3页Journal of Zunyi Medical University

摘　　要：目的检测STN抗原在产后出血中的表达,研究STN抗原与产后出血的关系,寻找引起产后出血的确切病因。方法通过HE染色、STN及cytokeratin(H)免疫组化染色,对19例尸检标本进行回顾性研究。结果10例临床诊断为产后出血的病例中,STN抗原表达阳性5例。其中,1例为弱阳性,且cytokeratin(H)表达呈阴性;其他4例为强阳性,且cytokeratin(H)表达呈阳性。HE染色均未观察到鳞状上皮、胎粪小体、胎脂及粘液等羊水有形成分。尸检明确诊断为羊水栓塞的2例病例,STN抗原、cytokeratin(H)表达均呈阳性,HE染色观察到胎脂或胎粪小体的存在。其他死因的7例病例,STN抗原表达均呈阴性,cytokeratin(H)表达阳性2例;HE染色也没有观察到羊水有形成分。STN抗原的阳性率:实验组与阳性对照组、阴性对照组比较有差异(P<0.05)。结论原因不明的产后出血,需检测羊水及胎粪的特征性成分——STN抗原,以明确是否为羊水进入母体血循环引起凝血功能障碍而导致的出血。Objectives To detect the expression of Sialyl Tn antigen in postpartum hemorrhage,study on the relationship between Sialyl Tn antigen and postpartum hemorrhage and seek the definite cause of disease arousing postpartum hemorrhage. Methods The 19 autopsy cases were studied using HE stainining and immuohistochemistry staining. Results Among the 10 cases diagnosed as postpartum hemorrhage, 5 cases expressed the STN antigen： one was weakly positive and not expressed cytokeratin （H） antigen;the other four eases were strong positive and expressed cytokeratin （H） antigen. There did not observe squamous epithelium, meconium body, fetal fat and mucus through HE staining among all 5 cases. Among the 2 cases clear diagnosed as amniotic fluid embolism by autopsy and HE staining,the expression of STN antigen and cytokeratin （H） antigen were positive, and have be found the fetal body fat or the meconium in HE staining. Other causes of the 7 cases,the expression of STN antigen were negative,2 cases expressed the cytokeratin （H） antigen,and did not be found amniotic fluid components in HE staining. The positive rate of STN antigen has statistics significance by comparing the difference between the experimental group and the positive control group and the negative control group （P〈0.05）. Conclusions In the unidenthqed postpartum hemorrhage,need to check the STN antigen which is the characteristic component of the amniotic fluid and the meconium,and identify whether hemorrhage was leaded by blood clotting barrier which was caused by the amniotic fluid entered maternal blood circulation.

关 键 词：STN抗原 产后出血 羊水栓塞 免疫组织化学 

分 类 号：R714.46[医药卫生—妇产科学]