荧光定量PCR技术对1235例NGU病原体基因检测结果分析  

Detection and Analysis of the gene of causative agents of 1235 cases of non-gonococcal urethritis by fluorescence quantitative PCR

作　　者：谭炳添[1] 周晓兰[1] 顾艳[1] 

机构地区：[1]遵义医学院第五附属(珠海)医院检验科,广东珠海519100

出　　处：《遵义医学院学报》2008年第4期358-360,共3页Journal of Zunyi Medical University

摘　　要：目的用实时荧光定量PCR(FQ-PCR)技术准确定量检测非淋菌性尿道炎(NGU)中解脲支原体(UU)、沙眼衣原体(CT)基因,进一步了解UU、CT感染情况,为临床治疗提供依据。方法采用FQ-PCR技术检测标本1235份。结果UU阳性率是27.5%,其中男性UU阳性率是12.4%,女性UU阳性率是42.0%。CT阳性率是12.1%,其中男性CT率是11.2%,女性CT率是13.2%。UU感染率在男性、女性患者有极显著性差异(P<0.01),而CT感染率在性别之间无显著性差异(P>0.05)。结论实时荧光定量PCR检测UU、CT基因具有操作简单、反应时间短、重复性好、结果客观准确、敏感性和特异性好等优点,其结果对临床诊断、治疗有一定指导意义。Objective To detect quantitatively the gene of Ureaplasm urealyticum （UU） and Chlamydia trachomatis （CT） in non--gonococcal urethfitis by technology of fluorescence quantitative polymerase chain reaction （FQ-PCR）,to understand further infections condition of UU and CT ,and to provide clinical treatment evidence to the disease. Methods Detection 1235 speciments by technology of FQ-PCR. Results the positive of UU was 27.5% of all these;male posiaive was 12.4%, and female was 42.0%0 the positive of CT was 12.1% of all these male posiaive was 11.2% ,and female was 13.2%. Infectious rates of UU had obvious statistics difference between males and females,but CT hadn＇t. Conclusions The fluorescence quantitative PCR assays showed advantages which were mainpulated simply,time--saving,more extra,more sensitive and more spectific in detecting CT and UU. FQ-PCR had directive meaning for dagnosis and treatmeat.

关 键 词：非淋菌性尿道炎(NGU) 沙眼衣原体 解脲支原体 实时荧光定量PCR 

分 类 号：R695[医药卫生—泌尿科学]