甲状旁腺激素PTH 1-34对成骨细胞的机械应答的影响  

The Effect of PTH 1-34 on Osteoblast-like Cells under Supra-threshold Loading

作　　者：刘云启[1] 张卫群[2] 

机构地区：[1]山东滨州医学院附属医院,山东滨州256600 [2]山东大学附属山东省立医院,山东济南250021

出　　处：《现代生物医学进展》2008年第10期1874-1876,共3页Progress in Modern Biomedicine

摘　　要：目的:探讨PTH对成骨细胞在机械载荷状态下的功能影响。方法:人成骨样细胞株MG63复苏培养,采用163mOsm低渗液作为刺激源,实验分为三组,A组为单纯用DMEM培养基培养;B组为低渗液对成骨细胞分别作用0.5h、2h、4h和6h;C组为低渗处理同期加入hPTH1-34(20ng/L)。检测细胞内钙离子浓度[Ca2+]i、细胞凋亡指数以及增殖活力的变化。所得结果采用单因素方差分析法。结果:随着培养时间延长,[Ca2+]i升高,以B组升高最为明显,(与A组比较p<0.05),A组和C组比较p>0.05;细胞凋亡指数在B组4h和6h为最高(29.38±0.336;54.87±0.781),明显高于A组和C组,且与两组相比均p<0.05,C组4h凋亡率低于A组(p<0.05),其它时间组统计学比较p>0.05。细胞增殖活力随着培养时间增加逐渐增加,与A组和C组同期相比,B组较低,且p<0.05。结论:过强的低渗牵张作用对细胞有损害作用,[Ca2+]i降低,凋亡率增加且降低增殖活性;PTH通过维持[Ca2+]i浓度,对细胞的功能具有保护作用。Objective： To investigate the effect of hPTH 1-34 on osteoblast-like cells MG63 under supra-threshold loading. Methods： Human osteoblast-like MG63 cell lines were incubated in 6 well plates. The 163 mOsm hypotonic solution was applied as the stimulation. Then the cell lines were divided into three groups： group A： cultured in DMEM medium; group B： treated with hypotonic solution for 0.5 h, 2 h, 4 h and 6 h respectively; group C： hPTH1-34 was added into the hypotonic solution for 0.5 h, 2 h, 4 h and 6 h respectively. Data was obtained from the study and then analyzed with SPSS （version 11.0 ） and one-way ANOVA test. Values of p〈0.05 were accepted as statistically significant. Results： Intracellular calcium level in Group B was the highest among the three groups, compared with group A and C, p〈0.05. However, there were no significant differences between group A and group C, p〉0.05. The apoptosis index in group B at 4 h and 6 h was higher than that in group A and C （vs. group A and C, p〈0.05）. The apoptosis radio in group C was lower than that in group A, p〈0.05. The proliferation activity in the three groups increased, but in group B it was lower than that in group A and C, p〈0.05. Conclusions： Supra-threshold stimuli suppressed intracellular calcium to drift into extracellular matrix and proliferation and promoted apoptosis; however hPTH1-34 protected osteoblast-like MG63 cell lines from supra-threshold loading through maintaining endocellular calcium level.

关 键 词：hPTH1-34 成骨样细胞 过载荷 

分 类 号：R977[医药卫生—药品]