植物生长调节剂对白芦笋花药培养来源试管苗生根诱导的研究  

A Study on the Effects of Several Plant Growth Regulators on Rooting of White Asparagus(Asparagus officinalis L.)Test-tube Seedling from Anther Culture

作　　者：林宗铿[1] 陈振东[1] 蔡坤秀[1] 杨俊杰[1] 张天翔[1] 

机构地区：[1]福建省热带作物科学研究所,福建漳州363001

出　　处：《江西农业大学学报》2008年第4期651-655,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：福建省科技厅科技攻关重点项目(2002N033);福建省自然科学基金项目(B0510035)

摘　　要：为建立白芦笋单倍体育种技术体系以解决白芦笋新品种资源贫乏问题,以白芦笋花药培养经愈伤组织途径分化而来的绿芽的茎尖为外植体,研究培养基中不同生长素(IBA、IAA、NAA)和植物生长延缓剂(S3307)对白芦笋试管苗生根的影响。结果表明:IBA单独使用可诱导试管苗生根,但有效生根率低;复合使用IBA、NAA和IAA3种生长素能提高试管苗的有效生根率;较高培养温度(25±1)℃下,在生根培养基中添加植物生长延缓剂S3307 0.1 mg/L,能提高试管苗的有效生根率;白芦笋花药培养来源试管苗适宜的生根培养基为1/2MS+KT0.1 mg/L+NAA0.1 mg/L+IAA0.5 mg/L+IBA2.0 mg/L+S3307 0.1 mg/L。Experiments were conducted on several plant growth regulators （tBA, NAA, IAA and S3307 ） to study the rooting of Asparagus officinalis L. test - tube seeding from anther. The results indicated that IBA itself could induce test - tube seedlings＇ rooting, but the availability rooting rate was low. And the availability rooting rate was promoted by admixture of IBA, NAA and IAA. At the higher temperature （25 ± 1 ） ℃, the plant growth retardant S3307 0.1 mg/L could increase the availability rooting rate. The optimum culture medium was 1/2MS ＋ KT 0. 1 mg/L ＋ NAA 0.1 mg/L ＋ IAA 0.5 mg/L ＋ IBA 2.0 mg/L ＋ S3307 0.1 mg/L.

关 键 词：白芦笋 花药培养 试管苗 生根诱导 植物生长调节剂 

分 类 号：Q945.3[生物学—植物学] S644.6[农业科学—蔬菜学]