粒细胞-巨噬细胞集落刺激因子与生长抑素融合表达基因疫苗的免疫对断奶仔猪某些代谢物浓度的影响  

Effect of Oral Fusion-Expression Plasmid Containing GM-CSF and SS on Metabolite Concentration of Weaned Piglets

作　　者：舒邓群[1] 陈荣达[1] 茆达干[2] 吴志敏[1] 杨利国[3] 

机构地区：[1]江西农业大学动物科技学院,江西南昌330045 [2]南京农业大学动物繁育研究所,江苏南京210095 [3]华中农业大学动物科技学院,湖北武汉430070

出　　处：《江西农业大学学报》2008年第4期693-696,共4页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金(30270959)

摘　　要：选择40头断奶的长×大二元杂交仔猪，随机分为4组，每组10头，公母各半。其中1组为对照组。2～4组分别口服以减毒沙门氏菌为载体的基因疫苗pcS／2SS、pGM—CSF／SS和pCM—CSF＋pcS／2SS。研究这些基因疫苗对断奶仔猪血浆代谢物浓度的影响。结果显示。对照组和pcS／2SS基因疫苗免疫组仔猪血浆葡萄糖浓度没有明显变化，pGM—CSF＋pcS／2SS免疫组呈现下降的趋势。融合表达质粒pGM—CSF／SS的免疫则可提高血浆葡萄糖的浓度；血浆游离脂肪酸的分泌水平各免疫组间无明显差异；血浆尿素氮的浓度pGM—CSF／SS免疫组有上升的趋势。在第2周、4周和6周血浆尿素氮的浓度高于其它3个组（P〉0．05）。pcS／2SS免疫组则随免疫时间的延长而逐渐下降。对照组和pGM—CSF＋pcS／2SS免疫组一直保持比较平稳的态势。结果表明。GM—CSF提高了SS基因疫苗的免疫效果。pGM—CSF／SS要优于pGM—CSF＋pcS／2SS共同免疫。A total of 40 weaned Landrace × Yorkshire crossbred piglets were divided into 4 groups to analyze metabolite concentration in piglet plasma. All the piglets of the 4 groups were given twice immunizations at intervals of 2 weeks. Group 1 as control was immunized with 0.85 % salt solution. Group 2 - 4 were orally inoculated with pcS/2SS （ group 2）, pGM - CSF/SS （ group 3 ） and pGM - CSF ＋ pcS/2SS （ group 4） delivered by attenuated Salmonella as carrier at a dose of 4 × 10^10CFU per piglet, respectively. The results demonstrated that immunization with pcS/2SS DNA vaccine did not affect plasma glucose （Glu） concentration, while Glu concentration of pGM - CSF/SS group increased after immunization. Plasma noncsterified fatty acids （ NEFA） concentrations among the 4 groups were not significantly different. After immunization with pGM - CSF/SS DNA vaccine, blood urea nitrogen （BUN） concentration exhibited declination. Their concentrations at 2nd,4th and 6th week were higher than those of the other groups. The concentrations in the control group and pGM - CSF ＋ pcS/2SS groups retained stable during the whole period. These results indicated that GM - CSF can enhance immunization effect on SS DNA vaccine. The immune response of pGM - CSF/SS is higher than that of co - immunization of pGM - CSF ＋ pcS/2SS.

关 键 词：粒细胞-巨噬细胞集落刺激因子 生长抑素 融合表达质粒DNA疫苗 代谢物 

分 类 号：S852.5[农业科学—基础兽医学]