氟环唑外消旋体在纤维素-三(3,5-二甲基苯基氨基甲酸酯)手性固定相上的高效液相色谱法拆分  

Chiral separation of racemic epoxiconazole on cellulose tris (3,5-dimethylphenylcarbamate) chiral stationary phase using high performance liquid chromatography

作　　者：韩小茜[1] 魏燕[1] 刘艳华[1] 常静[1] 仇伟[1] 陈峰[1] 

机构地区：[1]兰州交通大学化学与生物工程学院,甘肃兰州730070

出　　处：《色谱》2008年第5期631-633,共3页Chinese Journal of Chromatography

基　　金：甘肃省科技厅自然科学基金项目(No.32S042-B25-020);甘肃省科技攻关计划项目(No.2GS064-A52-036-06);兰州交通大学"青蓝"人才工程基金项目

摘　　要：在纤维素-三(3,5-二甲基苯基氨基甲酸酯)(CDMPC)手性固定相上,分别采用正相、反相及极性有机相色谱模式对氟环唑外消旋体进行了拆分,并考察了流动相组成在手性识别中对手性分离的影响。氟环唑在Chiralcel OD-H手性色谱柱(填充CDMPC手性固定相)上采用反相色谱模式,以甲醇-水(体积比为80∶20)为流动相,获得了最佳的拆分,其两对对映异构体的分离度Rs分别为1.64和6.50。The two pairs of enantiomers of epoxiconazole were resolved using normal phase,reversed-phase and polar organic solvent high performance liquid chromatography on cellulose tris（3,5-dimethylphenylcarbamate）（CDMPC）chiral stationary phase,separately.The effects of different mobile phase compositions on the retention factor,separation factor and resolution in the chiral separation of epoxiconazole were investigated.It can be baseline separated on a Chiralcel OD-H column packed with CDMPC chiral stationary phase when methanol-water（80∶20,v/v）was used as the mobile phase,the resolutions of the two pairs of enantiomers were 1.64 and 6.50,respectively.

关 键 词：纤维素-三(3 5-二甲基苯基氨基甲酸酯)手性固定相 正相色谱 反相色谱 极性有机相色谱 对映异构体分离 氟环唑外消旋体 

分 类 号：O658[理学—分析化学]