酶联免疫吸附法测定血浆中芍药苷浓度  

Enzyme-linked Immunosorbent Assay for the Determination of Paeoniflorin in Plasma

作　　者：蒋慧娟[1] 陈亮[1] 孙洋[1] 徐强[1] 陈婷[1] 

机构地区：[1]南京大学生命科学学院医药生物技术国家重点实验室,南京210093

出　　处：《分析化学》2008年第9期1238-1240,共3页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金重大研究计划资助项目(No.90209040)

摘　　要：建立了间接竞争酶联免疫吸附法测定血浆中芍药苷浓度。在10～200μg/L浓度范围内,线性关系良好,线性回归方程为y=-0.1721logC+1.9645,相关系数0.9927;回收率为89%～102%。运用本方法测定了大鼠一次灌胃后血浆中的芍药苷浓度。比较了分别给药芍药苷单体及中药复方四逆散提取物后,血浆中的芍药苷浓度。研究结果表明:四逆散配伍组方后,芍药苷的生物利用度较低。A method was developed for the determination of paeoniflorin in plasma by competitive enzyme- linked immunosorbent assay（ELISA）. The artifical antigen was used as immunogen and applied in New Zeal- and rabbits to get the anti-paeoniflorin antibodies. Hapten coupled with OVA was used as coating antigen. The linear concentrations ranged from 10 μg/L to 200 μg/L and the calibration curve was Y = -0. 1721 logC ＋ 1. 9645. Recoveries for the standard addition paeoniflorin to blank palsma were 89% - 102%. The method has been successfully applied to determine the concentration of paeoniflorin in rat plasma. After an oral administra- tion of the Si-Ni-San decoction, the bioavailability of paeoniflorin was lower than a single dose of paeoniflorin.

关 键 词：芍药苷 四逆散 酶联免疫吸附法 

分 类 号：R285[医药卫生—中药学]