邻苯二甲醛衍生荧光法测定牛奶中的苄青霉素残留  

Determination of Benzylpenicillin Residue in Milk by Fluorescent Spectrophotometry with o-Phthalaldehyde Derivatization

作　　者：李佳[1] 陈冠华[1] 郝庆红[1] 杨磊[1] 

机构地区：[1]河北农业大学生命科学学院

出　　处：《分析化学》2008年第9期1285-1288,共4页Chinese Journal of Analytical Chemistry

基　　金：河北农业大学博士(博士后)、回国人员科研基金资助项目

摘　　要：长期饮用含高浓度苄青霉素残留的牛奶可带来严重的健康问题,建立一种可对其进行灵敏检测的方法是必要的。苄青霉素在酸性条件下的水解产物之一是青霉胺,与邻苯二甲醛衍生后可生成具有强荧光的1-硫代2-烷基异吲哚衍生物,据此建立了荧光分光光度法测定苄青霉素的新方法。在水解时间为1h、衍生反应体系pH为10.0、衍生试剂用量为40μL、衍生反应时间为15min的最佳测定条件下,线性动态范围为2.0～500μg/L,检出限为1.3μg/L,样品的平均加标回收率为89.1%,方法可满足我国对牛奶中苄青霉素残留的限量要求。Penicillamine is one of the hydrolyzates of benzylpenicillin under acid condition and can be derivat- ized as hyperfluorescent 1-thio 2-aldyl iso-indole with o-phthaldehyde. A new fluorescent spectrophotometric assay based on the derivative reaction has been developed to determine benzylpenicillin residues. Under the optimum conditions such as hydrolyzation time of 1 h, pH 10.0, derivative reagent of 40 μL and reaction time of 15 min, the linear dynamic range is 2.0 -500 μg/L, the detection limit is 1.3 μg/L, and the average re- coveries of spiked sample is 89.1%. The requirement for benzylpenicillin residue in milk in national standards can be met with the assay.

关 键 词：苄青霉素残留 邻苯二甲醛 衍生 荧光分光光度法 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]