东方百合组培快繁试验  

Study on Cultivation Technique in Fast Propagation of Tissue Culture in Oriental Lily

作　　者：樊兰瑛[1] 高宏樟[2] 郭常莲[1] 

机构地区：[1]山西省农业科学院农业资源综合考察研究所,山西太原030006 [2]山西省农业科学院现代农业研究中心,山西太原030031

出　　处：《山西农业科学》2008年第9期29-32,共4页Journal of Shanxi Agricultural Sciences

基　　金：山西省科技攻关项目(2006031034)

摘　　要：采用东方百合Tiber品种的多种外植体分别进行了组培试验,结果表明,Tiber百合各外植体形成愈伤组织的能力有差异,其能力大小依次为:小鳞茎>花托>鳞片>叶柄>叶片;Tiber百合最佳诱导分化培养基为MS+2,4-D 1.0 mg/L+6-BA 0.5 mg/L,继代最佳培养基为MS+6-BA 0.1 mg/L+NAA 0.1mg/L,生根培养基为1/2 MS+NAA 0.5 mg/L。Oriental lily of the test using a variety of species Tiber explant respectively conducted experiment group, the resuits showed that the Tiber Lily explant callus formation of the difference between the ability. Its ability to size folIowed by small bulb, the receptacle, scales, petiole, and leaf was minimum in differentiation explants. The best medium for induction and bud differentiation of oriental lily was MS with 2,4 - D 1.0 mg/L and 6 - BA 0.5 mg/L. The best medium for proliferation was MS with 6 - BA 0. 1 mg/L and NAA 0.1 mg/L. 1/2 MS supplemented with NAA 0.5 mg/L was suitable for rooting.

关 键 词：东方百合 组培 快繁 

分 类 号：S644.103.8[农业科学—蔬菜学]