βGlcY影响油菜小孢子胚胎发生起始分裂  

Effect of βGlcY on microspore initial division during embryogenesis in Brassica napus (Topas) cv L

作　　者：汤行春[1] 何宇清[2] 孙蒙祥[2] 王征荣[1] 

机构地区：[1]湖北大学生命科学学院,湖北武汉430062 [2]武汉大学生命科学学院,湖北武汉430072

出　　处：《湖北大学学报（自然科学版）》2008年第3期298-301,共4页Journal of Hubei University：Natural Science

基　　金：国家"973"项目(2007CB108700);湖北省自然科学基金(2006ABA018)资助项目

摘　　要：以油菜小孢子为材料,研究不同浓度的βGlcY对小孢子胚胎发生起始分裂的影响,结果表明,βGlcY对油菜小孢子胚胎发育起始细胞分裂的抑制作用是浓度依赖的行为.βGlcY浓度为50μmol/L时,小孢子的分裂完全受到抑制.细胞学研究发现,βGlcY与AGPs结合后影响了小孢子的活性,推测βGlcY可能诱导了小孢子的程序化死亡.Experiments were employed to investigate effect of AGPs on embryogenesis in Brassica napus （Topas） cv L. The results showed that the addition of βGIcY notably disturbed microspore embryogenesis in a concentration-dependent manner. The initiation of microspore embryogenesis was clearly inhibited by 30 μmol/L βGlcY and completely inhibited by 50 μmol/L βGlcY. Cytological observation has determined that combination of βGlcY with AGPs clearly influence the viability of microspores, and that βGlcY/AGPs interaction could induce rnicrospore program death. Thus, AGPs appear to play a role in the initiation of Brassica microspore embryogene＇sis.

关 键 词：油菜 小孢子 胚胎发生 阿拉伯半乳糖蛋白 

分 类 号：Q944.46[生物学—植物学]