花生种子总RNA提取的一种有效方法  

An Efficient Method of Total RNA Extraction from Peanut Seeds

作　　者：任增凯[1] 禹山林[2] 杨庆利[2] 潘丽娟[2] 陈明娜[2] 郑强 

机构地区：[1]青岛农业大学植物科技学院,山东青岛266109 [2]山东省花生研究所,山东青岛266100 [3]乳山市农技站,山东乳山264500

出　　处：《花生学报》2008年第3期20-23,共4页Journal of Peanut Science

基　　金：国家重点基础研究发展计划资助项目(2007CB116212)

摘　　要：从植物中提取RNA的方法有多种,由于花生种子富含脂肪、蛋白质、多糖和酚类化合物,有些方法并不适于花生种子RNA的提取。本文设计了一种从花生种子中分离高质量总RNA的方法,并提取了花生的根、茎、叶、种子等不同器官的RNA。结果证明,利用该方法提取的花生种子总RNA质量较高,完全能满足后续的分子生物学研究。同样也适于花生根、茎、叶总RNA的提取。Many methods for the extraction of RNA from plant tissues haven been developed at present. Because peanut seeds are rich in lipid, protein, polysaccharide and phenolic compounds, some methods that usually used are not appropriate for the extraction of RNA from peanut seeds. A highly efficient method of total RNA extraction from peanut seeds was developed. We applied the method to isolate total RNA from seed, root, stem and leaf of peanut. The results showed that the RNA obtained from peanut seeds with this method had high quality and could be used in molecular biological research. The method also could be used in the isolation of total RNA from root, stem and leaf.

关 键 词：花生种子 RNA提取 方法 

分 类 号：S565.203[农业科学—作物学] Q78[生物学—分子生物学]