阿米替林对大鼠静脉局部麻醉效应的半数有效浓度  

Median effective concentration of amitriptyline for intravenous regonal anesthega in rats

作　　者：康仙慧[1] 宋莉[1] 柴云飞[1] 杨邦祥[1] 杨帆[1] 上官王宁[2] 刘慧[1] 

机构地区：[1]四川大学华西医院麻醉科,成都市610041 [2]温州医学院附属第二院麻醉科

出　　处：《中华麻醉学杂志》2008年第7期626-629,共4页Chinese Journal of Anesthesiology

摘　　要：目的探讨阿米替林对大鼠静脉局部麻醉效应的半数有效浓度（EC50）。方法健康雄性大鼠90·只，体重190～240g，随机分为3组（n=30）：阿米替林组、布比卡因组和利多卡因组。将大鼠鼠尾均分成上段（近心端）、中段、末段（远心端）三部分并做标记。尾静脉穿刺置入套管针并接肝素帽，用驱血带从尾尖驱血至鼠尾上段和中段交界处，于驱血带上缘处固定止血带，随后各组于10s内分别注入规定浓度的阿米替林、布比卡因和利多卡因各0．5ml，均以0．9％生理盐水稀释，10min后松开止血带。采用序贯法进行实验，阿米替林组第1只大鼠尾静脉注射0．05％阿米替林，相邻浓度比值为1．414；布比卡因组第1只大鼠注射0．03％布比卡因，相邻浓度比值为1．667；利多卡因组第1只大鼠注射0．08％利多卡因，相邻浓度比值为1．250。计算各组的EC50和95％可信区间。于给药前1h（基础状态）、给药后3min和2d时测定甩尾反应时间（TFL）。观察大鼠是否出现局麻药神经毒性反应（烦躁不安、惊厥、死亡等）和鼠尾局部组织损伤。结果阿米替林组、布比卡因组及利多卡因组的EC50分别为0．111％（95％可信区间0．092％～0．133％）、0．058％（95％可信区间0．048％～0．078％）及0．129％（95％可信区间0．103％～0．160％）；与阿米替林组比较，布比卡因组EC。降低（P〈0．01），利多卡因组差异无统计学意义（P〉0．05）；与布比卡因组比较，利多卡因组EC50升高（P〈0．01）。与基础值比较，各组给药后各时点鼠尾上段TFL差异无统计学意义（P〉0．05），给药后3min时鼠尾中段TFL升高（P〈0．01），给药后2d时TFL差异无统计学意义（P〉0．05）。各组大鼠均未见神经毒性反应和鼠尾局部组织损伤。结论阿米替林产生的静脉局部麻醉效力低于布比卡因，与利多卡因相似。Objective To determine the median effective concentration （EC50） of amitriptyline for intravenous regional anesthesia （IVRA） in rats. Methods Ninety healthy male SD rats weighing 190-240 g were randomly divided into 3 groups （ n = 30 each） ： amitripryline group, bupivacaine group and lidocaine group. The rat＇s tail was divided into 3 epual parts： the proximal, middle and distal part. A 24 gauge needle was inserted into vena caudalis in the distal part. Esmarch bandage was applied around the tail from distal to proximal to expel blood from the tail and was removed after a tourniquet was applied between the proximal and middle part of the tail to occlude artery. 0.5 ml of amitriptyline, bupivacaine or lidocaine was injected into the tail vein immediately after the application of the tourniquet. Ten minutes after drug administration the tourniquet was released. The EC50 was determined by the up-and-down sequence method. The initial concentration of amitriptyline was 0.05% , the consecutive concentration-ratio was 1. 414 ; the initial concentration of bupivacaine was 0.03 %, the consecutive concentration-ratio was 1. 667 and the initial concentration of lidocaine was 0.08 %, the consecutive concentrationratio was 1.250. EC50 and 95% confidence interval were calculated. Tail-flick latency （TFL） was assessed at 1 h before （baseline） and at 3 min and 2 d after drug administration. Central nervous system toxicity （seizure, convulsion, death） and local tissue damage to the tail were also recorded. Results The EC50 for IVRA was 0.111% （95% CI, 0.092%-0.133%）in amitriptyline group; 0.058% （95% CI, 0.048%-0.078%） in bupivacaine group and 0. 129% （95% CI, 0.103%-0. 160% ） in lidocaine group respectively. The EC50 was significantly lower in bupivacaine group than in amitriptyline and lidocaine group. There was no significant difference in EC50 between amitriptyline and lidocaine group. The TFL measured at the proximal part of the tail was not significantly different between different t

关 键 词：阿米替林 麻醉 静脉 麻醉 局部 剂量效应关系 药物 

分 类 号：R614[医药卫生—麻醉学]