异丙酚对大鼠离体心脏缺血再灌注时NF—κB及iNOS的影响  

Effects of propofol on myocardial NF-κB and iNOS during myocardial ischemia-reperfusion in isolated rat hearts

作　　者：周春燕[1] 陈玉培[1] 

机构地区：[1]重庆医科大学附属第二医院麻醉科,400010

出　　处：《中华麻醉学杂志》2008年第7期648-650,共3页Chinese Journal of Anesthesiology

摘　　要：目的探讨异丙酚对大鼠离体心脏缺血再灌注时核因子κB（NF-κB）及诱导型一氧化氮合酶（iNOS）的影响。方法成年SD大鼠24只，体重200～300g，雌雄不拘，随机分为3组（n=8）：对照组（C组）、缺血再灌注组（I／R组）和异丙酚组（P组）。建立Langendorff离体心脏灌注模型，K-H液平衡20min后开始实验。C组灌注K-H液110min;I／R组灌注K-H液20min后，全心停灌30min，再灌注60min；P组用含50μmol／L异丙酚的K-H液灌注20min，全心停灌30min，再用含50μmol／L异丙酚的K-H液灌注60min。于平衡末、再灌注10min和60min时测定冠状动脉流出液心肌肌钙蛋白（cTnI）浓度；于再灌注60min时测定心肌SOD活性、MDA含量、iNOS活性及NF-κB、IκB的表达水平。结果与C组比较，I／R组再灌注期间冠状动脉流出液cTnI浓度升高，P组再灌注期间60min时升高（P〈0．05或0．01），I／R组心肌SOD活性降低，MDA含量增多，iNOS活性升高（P〈0．01），I／R组和P组心肌NF-κB表达升高，IκB表达降低（P〈0．05或0．01）。与I／R组比较，P组再灌注期间冠状动脉流出液cTnI浓度、心肌MDA含量、NF-κB表达、iNOS活性均降低，心肌SOD活性和IκB表达升高（P〈0．01）。结论异丙酚可抑制心肌NF-κB的激活，降低iNOS的活性，从而减轻大鼠离体心脏缺血再灌注损伤。Objective To investigate the effects of propofol on myocardial nuclear factor kappaB（NF-κB） and inducible nitric oxide synthase （iNOS） during myocardial ischemia-reperfusion （I/R） in isolated rat hearts. Methods Twenty-four adult SD rats of both sexes weighing 200-300 g were randomly divided into 3 groups （ n = 8 each） ： group Ⅰ control （C） ; group Ⅱ I/R and group Ⅲ propofol ＋ I/R （P）. The animals were anesthetized with intraperitoneal urethane 1.0 g/kg and their hearts were excised. The aorta was cannulated and the hearts were retrogradely perfused with oxygenated K-H solution in a Langendorff apparatus for 20 min. In group C the hearts were continuously perfused with K-H solution for 110 min. In I/R group after 20 min stabilization the hearts were made globally ischemic for 30 min followed by 60 min reperfusion. In group P the hearts were perfused with K-H solution containing 50 μmol/L propofol for 20 min, the hearts were then made globally ischemic for 30 min followed by 60 min reperfusion with K-H solution containing 50 μmol/L propofol. Cardiac troponinI （cTnI） concentration in coronary effluent was measured at the end of 20 min stabilization before myocardial ischemia （baseline） and at 10 min（T1 ） and 60 min（T2） of reperfusion. Myocardial specimen was obtained from left ventricle at 60 min of reperfusion for determination of MDA content, SOD and iNOS activity, the expression of NF-κB and IκB （by immuno-histochemistry）. Results The cTnI concentration in coronary effluent was significantly higher at T1 and T2 in group I/R and at T2 in group P than in group C. The NF-κB expression was significantly higher while IκB expression was lower in I/R and P group than in group C. iNOS activity was higher in I/R group than in control group but there was no significant difference in iNOS between group P and C. Propofol administered before and after myocardial ischemia significantly attenuated I/R-induced changes listed above. Conclusion Propofol can attenuate my

关 键 词：二异丙酚 心肌再灌注损伤 NF-κB 一氧化氮合酶 

分 类 号：R285.5[医药卫生—中药学]