小麦与叶锈菌互作过程中的H_2O_2与HR  

THE GENERATION OF H_2O_2 IN WHEAT HYPERSENSITIVE REACTION INDUCED BY LEAF RUST FUNGUS

作　　者：祁艳[1] 刘刚[1] 侯春燕[1] 王冬梅[1] 

机构地区：[1]河北农业大学生命科学学院,保定071001

出　　处：《分子细胞生物学报》2008年第4期245-254,共10页Journal of Molecular Cell Biology

基　　金：国家自然科学基金资助项目(No.30671244);植物生理学与生物化学国家重点实验室开放课题资助项目(No.PPB04006);河北省自然科学基金资助项目(No.303180;No.C2005000220;No.C2007000515);河北省应用基础研究计划重点基础研究项目(No.08965505D)

摘　　要：以小麦品种洛夫林10为材料,与叶锈菌生理小种165和260分别组成亲和组合和不亲和组合,以荧光探针DCFHDA标记H2O2的变化,并借助荧光显微镜原位地直观显现了叶锈菌侵染过程中小麦叶片中H2O2的动态变化。结果表明,亲和组合中H2O2爆发表现为单峰曲线,峰值出现在接种后12h;不亲和组合中表现为双峰曲线,峰值分别出现在接种后12h和20h,第二个峰比第一个高约一倍,且不亲和组合中H2O2爆发的强度远远大于亲和组合。另外,通过药理学试验,采用活体注射法对小麦叶片在接种叶锈菌小种260之前分别预注射抗氧化药物AsA、DTT以及NADPH氧化酶抑制剂DPI和咪唑,观察这些药物对不亲和组合中寄主叶片产生的H2O2和HR的影响。结果表明,抗氧化药物和NADPH氧化酶抑制剂均使寄主叶片中H2O2爆发的强度降低,表现在两个H2O2峰均受到抑制,其中第二个峰受抑制的程度更明显,同时寄主细胞发生HR的面积减小。H2O2 compatible and inco burst in the defense responses of Wheat （Triticum aestivum L.） infected with mpatible leaf rust fungus races （Puccinia recondita firstly. Cytofluorimetric analysis using H2O2 in wheat primary leaves. The the fluorescent probe DCFHDA f.sp. tritici） was investigated scrutinized the generation of results indicated that H2O2 burst could be induced by either compatible or incompatible leaf rust fungus. However, the kinetics of H2O2 burst in incompatible interaction were biphasic, and the peak of phase Ⅱ （20h after inoculation） was much higher than that of phase Ⅰ （12h after inoculation）, whereas, there only existed the weak and transient phase Ⅰ H2O2 accumulation at 12h after inoculation in compatible interactions. In addition, pharmacological experiments were carried out. Antioxidants （AsA, DTT） and inhibitors of the mammalian neutrophil NADPH oxidase （DPI, imidazole） were separately injected into wheat primary leaves before inoculation to study their effects on hypersensitive reaction （HR） and H2O2 production in incompatible interaction. It was observed that treatments of wheat leaves with AsA, DTT, DPI or imidazole obviously suppressed the two peaks of intracellular H2O2 burst,whereas the second peak was inhibited much more. Moreover, the area of hypersensitively dead cells was decreased in the four treatments. These results suggested an important role in H2O2 generation during wheat-leaf rust be involved in HR caused by leaf rust invading. fungus that NADPH oxidase may play interaction, and H2O2 burst may

关 键 词：小麦 叶锈菌 H2O2 HR(Hypersensitive reaction 过敏性反应) 

分 类 号：S435.121[农业科学—农业昆虫与害虫防治]