玉米离体根冠细胞凋亡的荧光显微观察(简报)  

APOPTOSIS IN DETACHED CORN ROOT CAP CELLS UNDER FLUORESCENT MICROSCOPE

作　　者：商闯[1] 贾银锁[1] 侯立白[2] 马春红[1] 

机构地区：[1]河北省农林科学院遗传生理研究所,石家庄050051 [2]沈阳农业大学农学院,沈阳110161

出　　处：《分子细胞生物学报》2008年第4期329-334,共6页Journal of Molecular Cell Biology

基　　金：河北省自然科学基金资助项目(C2006000744);国际科技合作资助项目(2006DFB02480)资助;河北省科学技术研究与发展计划资助项目(07297162D)~~

摘　　要：1969年Smedegard-Petersen和Nelson首次报道玉米小斑病菌毒素（Helminthosporium maydis toxin,简称Hm-毒素）时曾利用抑制种子根生长法和离体叶片法对Hm-毒素进行生物测定。以后Lim等人在小斑病菌T小种毒素（简称HMT毒素）的研究中提出了抑制根伸长法在抗病性鉴定和选育抗病品种方面有应用价值。但是，抑制根伸长法受种子个体间生活力差异的影响较大，不够理想，加上不同毒素制剂之间结果的差异大，在实际应用中存在很大困难。1982年，Apoptosis in detached corn root cap cells under fluorescent microscope was studied. Homokaryon maize B37 inbred lines was tested and the detached corn root cap cells with cell wall might be stained using Hoechst33342. Apoptosis was found under fluorescence microscope treated by culture filtrate of HMC toxin. The sensitivity of HMC toxin to C cytoplasm was greater than N cytoplasm and there was more resistance response in detached corn root cap cells of N cytoplasm than C cyto that special C cytoplasm plasm. Besides, apoptosis did not coincide at different processing times. These indicated disease response occurred in the course of apoptosis in detached corn root cap cells of maize led by HMC which may be collective result of nucleus and cytoplasm.

关 键 词：玉米小斑病菌C小种 毒素培养滤液 根冠细胞 凋亡 

分 类 号：S513[农业科学—作物学]