细胞周期蛋白依赖性激酶2活性与单纯疱疹病毒复制关系的研究  

The effect of cyclindependent kinase 2 on herpes simplex virus replication

作　　者：关泽红[1,2] 旭日干[1] 

机构地区：[1]内蒙古大学哺乳动物生殖生物学与生物技术教育部重点实验室,呼和浩特010021 [2]内蒙古医学院

出　　处：《中华微生物学和免疫学杂志》2008年第8期703-706,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然基金面上项目（30560009）

摘　　要：目的探讨细胞周期蛋白依赖性激酶（CDK）2在单纯疱疹病毒（HSV）复制中的作用。方法以能够诱导表达CDK2显性负突变体致使CDK2功能缺陷的人结肠癌细胞株HT29Tet-Ondn-CDK2为材料，用免疫共沉淀及放射自显影法测定HSV感染后不同时间CDK2活性，并进行HSV的增殖动力学分析。结果HSV感染6h后CDK2活性被诱导，9h达到最大；一步生长曲线结果表明在CDK2活性被诱导前HSV处于静止状态，CDK2活性被诱导后HSV即进入快速的生长复制阶段。结论CDK2是HSV复制所必需的，HSV通过诱导宿主细胞CDK2活性而促进本身的复制。Objective To explore the effect of cyclindependent kinase （CDK） 2 on the replication of herpes simplex virus （HSV）. Methods Dominant-negative CDK2 expressing cell line HT29Tet-On dn- CDK2 was used as host cells to determine the role of CDK2 in HSV replication. HT29Tet-On dn-CDK2 cells were infected with HSV. The activity of CDK2 was determined by the immunoprecipitate and autoradiograph before and after HSV infection. The growth curve of HSV was conducted. Results The CDK2 activity was induced after infection for 6 h and reached the highest level at 9 h. HSV replicated rapidly and productively after the CDK2 was induced. Conclusion CDK2 is required for the replication of HSV, and HSV replicates in host cells by inducing the CDK2 activity.

关 键 词：单纯疱疹病毒 细胞周期蛋白依赖性激酶2 病毒复制 

分 类 号：R3[医药卫生—基础医学]