p16^(INK4a)在早孕小鼠子宫内膜的表达及其对胚泡着床的影响(英文)  

Expression of p16^(INK4a) in mouse endometrium and its effect during blastocyst implantation

作　　者：杨环[1] 谢怡[1] 杨戎[1] 魏莎莉[1] 郗强[1] 

机构地区：[1]重庆医科大学生殖生理教研室,重庆400016

出　　处：《生理学报》2008年第4期547-552,共6页Acta Physiologica Sinica

基　　金：Natural Science Foundation of Chongqing Science and Technology Committee, China (2007BB5273)

摘　　要：本研究旨在检测肿瘤抑制基因p16INK4a(inhibitor of cyclin-dependent kinase4a)在早孕小鼠子宫内膜中的表达规律,探讨p16INK4a在小鼠胚胎着床过程中的作用。采用荧光定量PCR(FQ-PCR)和免疫组织化学方法分别检测未孕小鼠及孕小鼠第2、3、4、5、7天子宫内膜p16INK4a mRNA和蛋白的表达;子宫角注射p16INK4a抗体观察胚泡着床数。FQ-PCR结果显示孕小鼠子宫内膜组织p16INK4a mRNA的表达高于未孕小鼠,且随着妊娠天数的增加呈现表达逐渐增强的趋势,到妊娠第5天达到最高,后渐降。免疫组织化学分析显示p16INK4a蛋白在子宫内膜的表达规律与mRNA结果一致。子宫角注射p16INK4a抗体后胚泡着床数明显减少。以上结果提示,p16INK4a在妊娠早期子宫内膜持续表达,可能参与胚泡着床。The expression of tumor suppressor gene p16^INK4a in mouse endometrium during early pregnancy and its possible role in blastocyst implantation were investigated in the present study. Real-time fluorescent quantitative PCR （FQ-PCR） and immunohistochemistry were applied to detect p16^INK4a mRNA and protein expressions in endometrium of un-pregnant and pregnant mice on day 2, 3, 4, 5, 7, respectively. In addition, p16^INK4a antibody was injected into the horns of uteri in pregnant mice on day 3 and its effect during blastocyst implantation was detected in vivo. The higher expressions of p16^INK4a mRNA and protein were observed in pregnant mice compared with that in un-pregnant mice, with a steady increase from day 2 to day 5 and reaching the maximal level on day 5 of pregnancy and then decreasing. p16^INK4a antibody decreased the number of implanted blastocysts compared with that of saline-injected group. The results suggest that p16^INK4a may be associated with apoptosis of luminal epithelial cells and decidual cells, coordinating decidualization of endometrium and invasion of trophoblastic cells. Thus, we presume that p16^INK4a participates in the process of blastocyst implantation in mice.

关 键 词：胚胎着床 子宫内膜 P16^INK4A 实时荧光定量PCR 免疫组织化学 体内实验 

分 类 号：R714[医药卫生—妇产科学]