Characterization of the target DNA sequence for the DNA-binding domain of zinc finger protein 191  

作　　者：Haoyue Wang Ruilin Sun Guoxiang Liu Minghui Yao Jian Fei Hebai Shen 

机构地区：[1]Department of Chemistry, Life and Environmental Science College, Shanghai Normal University, Shanghai 200234, China [2]School of Life Science and Technology, Tongji University, Shanghai 200092, China [3]Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China [4]Graduate School of Chinese Academy of Sciences, Beijing 100049, China

出　　处：《Acta Biochimica et Biophysica Sinica》2008年第8期704-710,共7页生物化学与生物物理学报（英文版）

基　　金：This work was supported by grants from the National Basic Research Program of China （No. 2002CB713803）, the National Natural Science Foundation of China （No. 20573075） and the Science and Technology Commission of Shanghai Municipality （Nos. 06XD14014, 0752nm028, 07DZ22303, 06DZ05137 and 54319939）Acknowledgements We would like to thank Ms. Jiajuan Shen （School of Life Science and Technology, Tongji University, Shanghai, China） for her help in ordering reagents and experiment materials.

摘　　要：Studies on the DNA-binding properties of transcription factors are important in searching for the downstream genes regulated by these factors. In the present study, we report on the DNA-binding property ofa Cys2His2-type transcription factor, zinc finger protein 191 （Zfp191）, which has been newly found to play a significant role in mice. By constructing a fusion protein containing the DNA-binding domain of Zfp 191, we characterized target DNA by determining the protein＇s binding specificity and dependence on zinc. The data showed that the DNA-binding domain of Zfp191 can specifically bind to the TCAT repeat motif and that there is a cooperative effect among the target DNA＇s multiple binding sites. Furthermore, the binding reaction is dependent on zinc. This work provides a foundation for further studies on the role of Zfp191 in gene regulation and development.Studies on the DNA-binding properties of transcription factors are important in searching for the downstream genes regulated by these factors. In the present study, we report on the DNA-binding property ofa Cys2His2-type transcription factor, zinc finger protein 191 （Zfp191）, which has been newly found to play a significant role in mice. By constructing a fusion protein containing the DNA-binding domain of Zfp 191, we characterized target DNA by determining the protein＇s binding specificity and dependence on zinc. The data showed that the DNA-binding domain of Zfp191 can specifically bind to the TCAT repeat motif and that there is a cooperative effect among the target DNA＇s multiple binding sites. Furthermore, the binding reaction is dependent on zinc. This work provides a foundation for further studies on the role of Zfp191 in gene regulation and development.

关 键 词：zinc finger protein 191 DNA-binding protein MICROSATELLITE 

分 类 号：Q753[生物学—分子生物学]