Cloning and characterization of a flowering time gene from Thellungiella halophila  

作　　者：Qiaoyun Fang Jun Liu Zhengkai Xu Rentao Song 

机构地区：[1]The Key Laboratory of Gene Resource Utilization for Severe Diseases, Ministry of Education, Anhui Medical University, Hefei 230032, China [2]School of Life Sciences, Shanghai University, Shanghai 200444, China [3]Shanghai Key Laboratory of Bio-energy Crop, Shanghai University, Shanghai 200444, China

出　　处：《Acta Biochimica et Biophysica Sinica》2008年第8期747-753,共7页生物化学与生物物理学报（英文版）

基　　金：This work was supported by a grant from the National Natural Science Foundation of China （No. 30471119）Acknowledgements We would like to thank Dr. Xiongfei Ju for his help in preparing this manuscript.

摘　　要：Thellungiella halophila （T. halophila） （salt cress） is a close relative of Arabidopsis and a model plant for salt tolerance research. However, the nature of its later flowering causes some difficulties in genetic analysis. The FRIGIDA （FRI） gene plays a key role in the Arabidopsis vernalization flowering pathway, whose homolog in T. halophila may also be a key factor in controlling flowering time. In order to study the molecular mechanism of vernalization responses in T. halophila, a full length cDNA named ThFRI （Thellungiella halophila FRIGIDA） was isolated from the young seedlings of T. halophila by RT-PCR and RACE. The ThFRI cDNA was 2017 bp in length and contained an open reading frame encoding a putative protein of 605 amino acids. The ThFRI showed significant homology to AtFRI （74.5 % at the nucleotide level and 63.9% at the amino acid level）. To study its function, ThFRI cDNA was transformed into Arabidopsis thaliana, driven by CaMV 35S promoter. Transgenic plants expressing ThFRI exhibited late-flowering phenotype, which suggests that ThFRI is the funtional FRI homolog in T. halophila. The cloning and funtional characterization of the FRI homolog of T. halophila will faciliate further study of flowering time control in T. halophila.Thellungiella halophila （T. halophila） （salt cress） is a close relative of Arabidopsis and a model plant for salt tolerance research. However, the nature of its later flowering causes some difficulties in genetic analysis. The FRIGIDA （FRI） gene plays a key role in the Arabidopsis vernalization flowering pathway, whose homolog in T. halophila may also be a key factor in controlling flowering time. In order to study the molecular mechanism of vernalization responses in T. halophila, a full length cDNA named ThFRI （Thellungiella halophila FRIGIDA） was isolated from the young seedlings of T. halophila by RT-PCR and RACE. The ThFRI cDNA was 2017 bp in length and contained an open reading frame encoding a putative protein of 605 amino acids. The ThFRI showed significant homology to AtFRI （74.5 % at the nucleotide level and 63.9% at the amino acid level）. To study its function, ThFRI cDNA was transformed into Arabidopsis thaliana, driven by CaMV 35S promoter. Transgenic plants expressing ThFRI exhibited late-flowering phenotype, which suggests that ThFRI is the funtional FRI homolog in T. halophila. The cloning and funtional characterization of the FRI homolog of T. halophila will faciliate further study of flowering time control in T. halophila.

关 键 词：Thellungiella halophila VERNALIZATION FRIGIDA ThFRI Arabidopsis thaliana 

分 类 号：Q344.4[生物学—遗传学]