铜绿假单胞菌中一个新的吩嗪合成调节基因  

A novel transcriptional regulator of the phzA1 operon in Pseudomonas aeruginosa

作　　者：孔丽萍[1] 梁海华[1] 董兆麟[1] 段康民[1] 沈立新[1] 

机构地区：[1]西北大学生命科学学院,西部资源生物与现代生物技术教育部重点实验室,西安710069

出　　处：《微生物学报》2008年第9期1154-1159,共6页Acta Microbiologica Sinica

基　　金：国家自然科学基金(30470098);重大基础研究前期研究专项(2004CCA01700)~~

摘　　要：【目的】在次抑制浓度四环素条件下,研究铜绿假单胞菌phzA1操纵子的调节基因及调节途径。【方法】对转座突变库中phzA1操纵子表达发生变化的突变体,进行随机PCR、基因测序及比对,确定突变位点。并以发光杆菌的荧光素酶基因操纵子luxCDABE为报道基因,研究基因调节作用及调节路径。【结果】在两株突变体PAM0487和PAM0487R中phzA1操纵子的表达降低,这两株突变体的突变基因确定为假定钼元素转运蛋白调节子PA0487基因。【结论】PA0487是phzA1操纵子表达的一个新的正向调节子,并对密度感应系统相关基因的表达有调节作用。[Objective] The aim was to investigate the regulator ofphzA1B1C1D1E1Fl（phzA1） operon in Pseudomoas aeruginosa, [Methods] Mutants affecting phzA1 expression in the presence of subinhibitory concentrations of tetracyclin were selected by screening a transposon mutagenesis library, The transposon insertion sites in the mutants were determined by arbitrary PCR and subsequent PCR product sequencing, [Results] We found two mutants affecting the phzA1 operon expression. The transposon insertion sites in these two mutants were at the promoter region of gene PA0487 encoding a probable molybdenum transport regulator Some genes involved in quorum sensing were also down-regulated in the mutant. [Conclusion] PA0487 was involved in regulating the expression of phzA1, possibly through the quorum sensing system.

关 键 词：铜绿假单胞菌 phzA1基因 密度感应系统 四环素 

分 类 号：R378[医药卫生—病原生物学]