光合细菌利用工业有机废水产生5-氨基乙酰丙酸  

Production of 5-aminolevulinic acid from organic industrial wastewater by photosynthetic bacteria

作　　者：刘秀艳[1] 徐向阳[2] 叶敏[3] 项硕[4] 

机构地区：[1]杭州电子科技大学环境科学与工程研究所,杭州310028 [2]浙江大学环境与资源学院,杭州310029 [3]杭州市环境保护科学研究院,杭州310014 [4]浙江科技学院建筑工程学院,杭州310014

出　　处：《微生物学报》2008年第9期1221-1226,共6页Acta Microbiologica Sinica

基　　金：国家自然科学基金(40771100)~~

摘　　要：【目的】利用本实验室筛选的5-氨基乙酰丙酸(5-aminolevulinic acid,ALA)高产紫色非硫红假单胞菌株,以味精、柠檬酸、啤酒和豆制品生产废水作为底物,进行光合细菌利用废水产生ALA并去除化学需氧量(CODcr)的研究。【方法】光合细菌培养温度为30℃,光照强度为3000Lux,进行乙酰丙酸、甘氨酸、琥珀酸的添加与否和废水灭菌与否的处理,用比色法测定菌液光密度,ALA检测采用Ehrlich’s试剂分光光度检测法。【结果】在不添加乙酰丙酸(levulinic acid,LA)、甘氨酸和琥珀酸的条件下,菌株99-28的菌体生长在72～96h达到稳定期,ALA产量在96h最高,在4种废水中,味精废水的ALA产量最高,CODcr去除率也最高;添加LA、甘氨酸和琥珀酸显著提高ALA产量,但CODcr去除效果不好。废水不灭菌略微降低99-28菌株的生长和CODcr的去除能力,在添加LA、甘氨酸和琥珀酸的条件下的,ALA产量明显下降。ALA高产突变菌株L-1在有机废水中的生长状况、对有机废水的CODcr去除与菌株99-28表现一致,在不添加和添加LA、甘氨酸和琥珀酸的条件下,突变株L-1的ALA产量明显比菌株99-28高。【结论】本实验室筛选的紫色非硫红假单胞菌株能利用有机废水作为底物产生ALA并降解CODcr。[Objective] We used Rhodopseudomonas strains with high-yield of 5-aminolevulinic acid （ALA） to produce ALA from wastewater of producing monosodium glutamate, citric acid, beer, and soybean product. [Methods] Cultivation was carried out under anaerobic light condition （3000 Lux） at 30℃. For comparison, we tested the addition of levulinic acid （LA）, glycin and succinate to the substrate to increase the production of ALA, effect of sterilization of the wastewater for both strains. Cell mass concentration （OD660） and the content of ALA were determined with spectrophotometer. [Results] Without adding levulinic acid （LA）, glycin and succinate, the growth of strain 99-28 reached plateau after 72-96 h. The maxiam ALA production was obtained at 96 h. Both the yield of ALA and the Chemical Oxygen Demand （CODcr） removal rate of monosodium glutamate waster water were the highest in all tested wasterwaters. When LA, glycin and succinate were added, ALA production of strain 99-28 was significantly increased whereas the CODcr removal was adversely affected. Non-sterial wasterwater slightly reduced the growth and CODcr removal rate of strain 99-28, however the ALA production could be strongly reduced with the addition of LA, glycin and succinate. The growth and CODcr removal of mutant strain L-1 was similar with strain 99-28, but its ALA production was much higher than that of strain 99-28. [Conclusion] The Rhodopseudomonas strains screened in our laboratory can use organic wasterwater as substrates to produce ALA and remove CODcr.

关 键 词：5-氨基乙酰丙酸 光合细菌 工业有机废水 

分 类 号：X703[环境科学与工程—环境工程]