γ射线与LPS作用于小鼠巨噬细胞RAW264.7的生物学效应  

Biological effect of γ-rays and LPS on the murine macrophage cell line RAW264.7 in mice

作　　者：饶亚岚[1] 丛悦[1] 陈肖华[1] 董波[1] 李峰生[1] 张军权[1] 高玲[1] 毛秉智[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《细胞与分子免疫学杂志》2008年第9期850-852,856,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30400119)

摘　　要：目的:研究γ射线与LPS协同激活小鼠巨噬细胞RAW264.7的效应机制,以及γ射线与LPS诱导钙结合蛋白S100A8的表达及意义。方法:相差显微镜观察细胞形态;流式细胞计数方法检测细胞周期及活性氧介质(ROI)水平;Griess颜色反应测定细胞NO水平;实时定量RT-PCR方法检测细胞S100A8 mRNA水平的表达。结果:γ射线与LPS作用于RAW264.7细胞引起细胞形态改变,部分细胞出现非整倍性,少量细胞出现凋亡,胞内ROI水平、NO水平明显升高,S100A8分子mRNA水平明显升高,而且这些效应几乎都比γ射线或LPS单因素的作用要强。结论:γ射线与LPS协同诱导巨噬细胞激活是细胞周期改变、信使分子水平变化、炎症因子如S100A8的表达等多方面生物效应的综合结果,其中S100A8基因的表达与巨噬细胞功能状态密切相关。AIM： To explore the effect of activating the murine macrophage cell line RAW264.7 by both γ-rays and lipopolysacchadde （LPS） and to study the expression of calcium-binding protein S100A8 induced by γ-rays and LPS. METHODS： The RAW264.7 cells were observed by phase contrast microscope. The cell cycle and the level of reactive oxygen intermediates （ROIs） were detected by flow cytometry （FCM）. The production of NO was measured by colorimetric Griess reaction. The mRNA expression of S100A8 was recorded by real-time quantitative RT-PCR method. RESULTS： The exposure of RAW264.7 cells to γ-rays and LPS resulted in the morphological change of cells, the rise of cells number of aneuploid and apoptosis, and the rise of the level of ROI, NO and S100A8 mRNA. The effect of using both γ-rays and LPS was stronger than that of single γ-rays or LPS treatment. CONCLUSION： The mechanism of using both γ-rays and LPS for activating macrophages is owing to the various biological effects including the change of cell cycle, the change of the level of messenger molecules and the expression of inflammation factor such as S100A8. The expression of S100A8 gene s closely correlated with the function and state of macrophages.

关 键 词：巨噬细胞 Γ射线 LPS S100A8 

分 类 号：R392.1[医药卫生—免疫学]