抗人转录因子TFDP3和TFDP1多肽抗体的制备  

Preparation of the polypeptide antibody against human transcriptional factors TFDP1 and TFDP3

作　　者：田婵[1,2] 李云燕[1] 乔欢[1] 张毓[1] 陈慰峰[1] 

机构地区：[1]北京大学医学部免疫学系,北京100083 [2]中国疾病预防控制中心病毒病预防控制所,传染病预防控制国家重点实验室,北京100052

出　　处：《细胞与分子免疫学杂志》2008年第9期875-877,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家高技术研究发展计划(863)资助项目(2003AA215110)

摘　　要：目的:制备兔抗人转录因子TFDP3、TFDP1的多克隆抗体,并鉴定其反应性及特异性。方法:选择TFDP3和TFDP1差异较大肽段,利用人工合成多肽免疫家兔,采用真核表达载体瞬时转染HEK293细胞表达TFDP3和TFDP1真核蛋白,通过Western blot分析抗体的反应性和特异性。结果:通过氨基酸序列比对选择TFDP3 17-26,TFDP1 17-32氨基酸残基合成多肽,制备了兔抗人TFDP3、TFDP1多肽抗体,Western blot检测显示,抗TFDP3抗体可以特异性识别TFDP3,而抗TFDP1抗体既可识别TFDP1又可与TFDP3结合。结论:制备了兔抗人TFD31和TFDP1的多肽抗体,且TFDP3抗体与TFDP1无交叉反应。AIM： To prepare the polyclonol antibody against human transcriptional factors DP3 （TFDP3） and DP1 （TFDP1） and identify their reactivity and specificity. METHODS： Two totally different peptides between TFDP1 and TFDP3 were chosen as antigens to inoculate rabbits. The proteins from HEK293 cells were transiently transfcted with eukaryotic expression vectors of TFDP1 and TFDP3. The reactivity and specificity of antibodies were detected by Western blot. RESULTS： The amino acid residues of TFDP3 17-26 and TFDP1 17-32 were selected and synthesized to produce polypeptide antibodies. Western blot analysis demonstrated that anti-TFDP3 antibody could specifically recognize TFDP3 protein, while anti-TFDPl antibody could both recognize TFDP1 and bind TFDP3 protein. CONCLUSION： The polypeptide antibodies against TFDP3 and TFDP1 have been prepared. The anti-TFDP3 antibody has no cross-reaction with TFDP1.

关 键 词：TFDP3 TFDP1 多肽抗体 

分 类 号：R392.11[医药卫生—免疫学]