苯甲酸利扎曲普坦对皮质扩布性抑制及中脑导水管周围灰质c—Fos表达的影响  

Effects of rizatriptan on cortical spreading depression and c-Fos expression within periaqueductal grey

作　　者：李凤鹏[1] 于生元[1] 管维平[1] 石宏[2] 陈淑莉[2] 晋志高 吴士文[4] 董钊[1] 邱恩超[1] 刘若卓[1] 姜磊[1] 

机构地区：[1]解放军总医院神经内科,北京100853 [2]中国中医科学院针灸研究所 [3]煤炭总医院神经内科 [4]武警总医院神经内科

出　　处：《中华神经科杂志》2008年第9期624-627,共4页Chinese Journal of Neurology

基　　金：全军“十一五”计划课题资助项目（06MA256）;国家自然科学基金资助项目（30570264）

摘　　要：目的观察苯甲酸利扎曲普坦对大鼠皮质扩布性抑制（cortical spreading depression，CSD）和CSD引起中脑导水管周围灰质（periaqueductal grey，PAG）内c-Fos表达的影响。方法实验选用的SD大鼠随机分为3组，A组：氯化钾刺激组；B组：氯化钾刺激＋苯甲酸利扎曲普坦组；C组：氯化钠刺激组。对各组大鼠使用氯化钾（或氯化钠）刺激，以观察CSD出现的个数和波幅；第1次刺激2h后使用免疫组织化学法检测各组PAG内不同层面c-Fos阳性神经元。结果C组未记录到CSD。A组CSD个数（10．70±3．23）较B组（6．10±2．56）显著增多（t=-3．528，P〈0．01）；A组CSD波幅[17．33（95％CI 11．45-23．11）mV]较B组[11．82（95％CI 9．24-14．70）mV]显著增加（Z=-4．360，P〈0．01）。A组各层面c-Fos阳性细胞数较B、C组显著增多（P〈0．05）。结论苯甲酸利扎曲普坦对CSD有抑制作用，可使CSD引起PAG内神经元兴奋作用减弱。Objective To determine whether rizatriptan has an effect on cortical spreading depression （CSD） and c-Fos expression within periaqueductal grey （PAG） induced by CSD in rats. Methods The experimental SD rats were randomly divided into group A injected with KCl, group B KCl plus rizatriptan and group C NaCl. The number and amplitude of CSD were recorded after KCl or NaCl injection. C-Fos positive neurons of different layer were identified by the immunohistochemical technique 2 hours after the first injection of KCl or NaCl. Results There was no CSD in group C. The number of CSD in group A （ 10. 70±3.23 ） was significantly more than that in group B （ 6. 10±2. 56, t = - 3. 528, P 〈 0. 01 ）. The amplitude of CSD in group A （17.33 （95% CI 11.45--23. 11 ）mV） was significantly greater than that in group B （11.82 （95%CI 9. 24--14.70） mV, Z= -4.360, P〈0.01）. There were more c- Fos-like immnoreactive neurons in every layer in group A than in group C （P 〈0.01 ） and in group B （P 〈 0.05 ）. Conclusion Rizatriptan has an inhibitory effect on CSD, which might induce the headache through exciting the neurons in PAG.

关 键 词：水管周灰质 三唑类 大脑皮质 原癌基因蛋白质C-FOS 基因 fos 

分 类 号：R686[医药卫生—骨科学]