聚己内酯/纳米羟基磷灰石复合微球中的药物分布及体外释放(英文)  

DRUG DISTRIBUTION WITHIN POLY(ε-CAPROLACTONE)/HYDROXYAPATITE NANOPARTICLE COMPOSITE MICROSPHERES AND IN VITRO RELEASE

作　　者：王旭东[1] 王迎军[1] 魏坤[1] 赵娜如[1] 张淑花[1] 

机构地区：[1]华南理工大学材料科学与工程学院

出　　处：《硅酸盐学报》2008年第9期1225-1230,共6页Journal of The Chinese Ceramic Society

基　　金：国家自然科学基金(59932050,50572029,50472054);国家“973”(2005CB623902);广东省自然科学联合基金(04205786)资助项目

摘　　要：采用单乳化溶剂挥发法制备了聚己内酯[poly(ε-caprolactone),PCL]/纳米羟基磷灰石(nano-hydroxyapatite,n-HA)复合微球。使用两种具有不同水溶性的模型药物对硝基苯胺(p-nitroaniline)和罗丹明B(Rhodamine B,RhB),研究n-HA在复合微球中的作用。用扫描电子显微镜观察微球的表面形貌。通过紫外–可见光分光光度法计算药物载量和包封率。用共聚焦激光显微镜分析药物在微球中的分布。分别研究了PCL微球和PCL/HA复合微球的体外释放性质。复合微球可以持续释放药物4周以上,在前3d的突释后,其释放曲线符合Higuchi扩散方程。n-HA的加入使较亲水药物RhB在复合微球中分布更均匀,对较疏水药物对硝基苯胺则影响不明显。n-HA减少了载亲水药物的复合微球在前3d的突释,并减缓了其后的释放速率。结果表明:PCL/n-HA复合结构的材料有希望作为一种新的长效药物释放载体应用。Poly （ε-caprolactone） （PCL）/hydroxyapatite nanoparticle （n-HA） composite microspheres were prepared via a single emulsion solvent evaporation method. Two model compounds （p-nitroaniline and Rhodamine B（RhB）） with different water solubilities were used to investigate the effect of HA nanoparticles on composite microspheres. The microspheres morphology was observed by scanning electron microscope. The drug loading and encapsulation efficiency were calculated by ultraviolet （UV） drug detection. Drug distribution within the microsphere matrix was studied by a confocal laser scanning microscope. The in vitro drug release properties of both the neat PCL and PCL/n-HA composite microspheres were investigated. After 3 d of initial burst, composite microspheres could sustainedly release drugs over 4 weeks, which could be explained by Higuchi＇s diffusion equation. The HA nanoparticles did not exhibit a distinct difference for p-nitroaniline-loaded microspheres, but helped RhB to be distributed more evenly. For hydrophilic compound-loaded composite microspheres, the HA nanoparticles could decrease the initial burst of the first 3 d, followed by a slower release stage. These behaviors make PCL/n-HA composite materials promising as a new drug release material for long-term applications.

关 键 词：聚己内酯 纳米羟基磷灰石 复合微球 药物缓释 

分 类 号：TB332[一般工业技术—材料科学与工程]